Department of Genetics, Agricultural University, Dreijenlaan 2, 6703, HA Wageningen, The Netherlands.
Theor Appl Genet. 1992 Sep;84(7-8):803-11. doi: 10.1007/BF00227388.
The development of linkage maps with large numbers of molecular markers has stimulated the search for methods to map genes involved in quantitative traits (QTLs). A promising method, proposed by Lander and Botstein (1989), employs pairs of neighbouring markers to obtain maximum linkage information about the presence of a QTL within the enclosed chromosomal segment. In this paper the accuracy of this method was investigated by computer simulation. The results show that there is a reasonable probability of detecting QTLs that explain at least 5% of the total variance. For this purpose a minimum population of 200 backcross or F2 individuals is necessary. Both the number of individuals and the relative size of the genotypic effect of the QTL are important factors determining the mapping precision. On the average, a QTL with 5% or 10% explained variance is mapped on an interval of 40 or 20 centiMorgans, respectively. Of course, QTLs with a larger genotypic effect will be located more precisely. It must be noted, however, that the interval length is rather variable.
利用大量分子标记构建连锁图谱的发展，刺激了人们寻找定位数量性状基因座（QTL）的方法。Lander 和 Botstein（1989）提出的一种很有前途的方法，采用相邻标记对来获得与 QTL 存在相关的最大连锁信息，其范围限定在被封闭的染色体片段内。本文通过计算机模拟对该方法的准确性进行了研究。结果表明，检测至少能解释总方差 5%的 QTL 有一定的概率。为此，需要至少 200 个回交或 F2 个体的群体。个体数量和 QTL 基因型效应的相对大小是决定作图精度的重要因素。平均而言，解释方差为 5%或 10%的 QTL 分别映射在 40 或 20 厘摩的区间上。当然，具有较大基因型效应的 QTL 将更精确地定位。然而，必须注意的是，区间长度相当多变。
