Lorieux M, Reversat G, Garcia Diaz S X, Denance C, Jouvenet N, Orieux Y, Bourger N, Pando-Bahuon A, Ghesquière A
IRD, UMR 5096, Rice Genomics Unit, B.P. 64501, 34394 Montpellier Cedex 5, France.
Theor Appl Genet. 2003 Aug;107(4):691-6. doi: 10.1007/s00122-003-1285-1. Epub 2003 Apr 30.
Inheritance of resistance to cyst nematode (Heterodera sacchari) in Oryza sativa was investigated by inoculation tests with isolate 244 from Congo in segregating populations derived from hybridisation between O. sativa and its African sister cultivated species, O. glaberrima. We found that the resistance was controlled by one major gene, Hsa-1(Og), with codominance of susceptible and resistant alleles. To map Hsa-1(Og) on the rice genome, we pooled the data obtained from segregation of the resistance trait and microsatellite markers in three kinds of progeny: BC(1)F(3), BC(1)F(4), and pseudo-F(2) populations. Hsa-1(Og) was unambiguously located between Cornell University's RM206 and RM254 markers on chromosome 11. Two additional microsatellite markers derived from Monsanto publicly available sequences were found to be tightly linked to the Hsa-1(Og) gene. It is possible that numerous plant resistances to a pathogen in fact exhibit a codominant inheritance, possibly explaining misleading conclusions in several reports on resistance segregation.
通过用来自刚果的244号分离株对接种试验进行研究，探讨了水稻对囊肿线虫（Heterodera sacchari）的抗性遗传，该试验针对的是水稻与其非洲姊妹栽培种光稃稻杂交产生的分离群体。我们发现，抗性由一个主基因Hsa-1(Og)控制，易感和抗性等位基因呈共显性。为了将Hsa-1(Og)定位到水稻基因组上，我们汇总了从三种后代群体（BC(1)F(3)、BC(1)F(4)和假F(2)群体）中抗性性状分离和微卫星标记获得的数据。Hsa-1(Og)明确位于第11号染色体上康奈尔大学的RM206和RM254标记之间。发现另外两个源自孟山都公开序列的微卫星标记与Hsa-1(Og)基因紧密连锁。事实上，许多植物对病原体的抗性可能表现出共显性遗传，这可能解释了几份关于抗性分离的报告中得出的误导性结论。
