New Zealand Institute for Crop & Food Research, Private Bag 4704, Christchurch, New Zealand.
Theor Appl Genet. 1996 Apr;92(5):505-8. doi: 10.1007/BF00224551.
During the initial field evaluation of transgenic plants, it is usual to isolate them genetically from other plants of the same species. Several field experiments on potatoes, using transgenes as markers, have shown that transgene dispersal by pollen to other potato plants is limited and very unlikely at distances over 10 m. In a recent study in Sweden, a frequency of transgene-containing progeny of over 30% is reported from non-transgenic potato plants grown at distances of 10-1000 m from transgenic plants containing nptII and gus marker genes. Data from the Swedish study is discussed along with other relevant observations, and it is concluded that the high frequency of gene dispersal in that study results from a high frequency of false positives during PCR analysis of the nptII gene. From the data available in potato, it is concluded that a distance of 20 m is generally adequate for the initial field evaluation of transgenic potatoes containing novel gene constructs.
在转基因植物的初步田间评估期间，通常将它们与同一物种的其他植物在遗传上进行隔离。使用转基因作为标记的几项马铃薯田间试验表明，花粉向其他马铃薯植株的转基因扩散是有限的，在超过 10 米的距离极不可能发生。在瑞典最近的一项研究中，从距离含有 nptII 和 gus 标记基因的转基因植物 10-1000 米的非转基因马铃薯植株中，报告了转基因后代的频率超过 30%。瑞典研究的数据与其他相关观察结果一起进行了讨论，得出的结论是，在对 nptII 基因进行 PCR 分析过程中出现高假阳性率，导致该研究中转基因的高扩散频率。根据马铃薯中可用的数据，得出的结论是，对于含有新型基因构建体的转基因马铃薯的初步田间评估，一般 20 米的距离就足够了。
