Isolation and identification of Triticum aestivum L. em. Thell. cv Chinese Spring-T. peregrinum Hackel disomic chromosome addition lines.

Analyses of RFLPs, isozymes, morphological markers and chromosome pairing were used to isolate 12 Triticum aestivum cv Chinese Spring (genomes A, B, and D)-T. peregrinum (genomes S(v) and U(v)) disomic chromosome addition lines. The evidence obtained indicates that each of the 12 lines contains an intact pair of T. peregrinum chromosomes. One monosomic addition line, believed to contain an intact 6S(v) chromosome, was also isolated. A CS-7U(v) chromosome addition line was not obtained. Syntenic relationships in common with the standard Triticeae arrangement were found for five of the seven S(v) genome chromosomes. The exceptions were 4S(v) and 7S(v). A reciprocal translocation exists between 4S(1) and 7S(1) in T. longissimum and evidence was obtained that the same translocation exists in T. peregrinum. In contrast, evidence for syntenic relationships in common with the standard Triticeae arrangements were found for only one U(v) chromosome of T. peregrinum.; namely, chromosome 2U(v). All other U(v) genome chromosomes are involved in at least one translocation, and the same translocations were found in the U genome of T. umbellulatum. Evidence was also obtained indicating that the centromeric regions of 4U and 4U(v) are homoeologous to the centromeric regions of Triticeae homoeologous group-6 chromosomes, that the centromeric regions of 6U and 6U(v) are homoeologous to the centromeric regions of group-4 chromosomes, and that 4U and 4U(v) are more closely related overall to Triticeae homoeologous group-6 chromosomes than they are to group-4 chromosomes.