Department of Crop and Soil Sciences, Washington State University, 99164-6420, Pullman, WA, USA.
Theor Appl Genet. 1993 Jul;86(6):705-12. doi: 10.1007/BF00222660.
A map of the barley genome consisting of 295 loci was constructed. These loci include 152 cDNA restriction fragment length polymorphism (RFLP), 114 genomic DNA RFLP, 14 random amplified polymorphic DNA (RAPD), five isozyme, two morphological, one disease resistance and seven specific amplicon polymorphism (SAP) markers. The RFLP-identified loci include 63 that were detected using cloned known function genes as probes. The map covers 1,250 centiMorgans (cM) with a 4.2 cM average distance between markers. The genetic lengths of the chromosomes range from 124 to 223 cM and are in approximate agreement with their physical lengths. The centromeres were localized to within a few markers on all of the barley chromosomes except chromosome 5. Telomeric regions were mapped for the short (plus) arms of chromosomes 1, 2 and 3 and the long (minus) arm of chromosomes 7.
构建了大麦基因组的图谱，由 295 个位点组成。这些位点包括 152 个 cDNA 限制片段长度多态性（RFLP）、114 个基因组 DNA RFLP、14 个随机扩增多态性 DNA（RAPD）、5 个同工酶、2 个形态标记、1 个抗病性标记和 7 个特异扩增片段多态性（SAP）标记。RFLP 鉴定的位点包括 63 个使用克隆的已知功能基因作为探针检测到的位点。图谱覆盖 1250 厘摩（cM），标记之间的平均距离为 4.2cM。染色体的遗传长度范围从 124 到 223cM，与它们的物理长度大致一致。除了染色体 5 之外，所有大麦染色体的着丝粒都定位在几个标记内。短（+）臂的端粒区域被映射到染色体 1、2 和 3，长（-）臂的端粒区域被映射到染色体 7。
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