莫霍克同源框转录因子调控肌腱和掌侧板的分化。
The Mohawk homeobox transcription factor regulates the differentiation of tendons and volar plates.
作者信息
Onizuka Naoko, Ito Yoshiaki, Inagawa Masayo, Nakahara Hiroyuki, Takada Shuji, Lotz Martin, Toyama Yoshiaki, Asahara Hiroshi
机构信息
Department of Systems BioMedicine, National Center for Child Health and Development, 2-10-1 Okura, Setagaya, Tokyo, 157-0074, Japan,
出版信息
J Orthop Sci. 2014 Jan;19(1):172-80. doi: 10.1007/s00776-013-0485-z. Epub 2013 Oct 29.
BACKGROUND
Mohawk (Mkx) is a homeodomain-containing transcription factor that is expressed in various mesoderm-derived tissues, particularly in developing tendons. In this study, we investigate the exact expression pattern and functions of Mkx in forelimbs.
METHODS
We analyzed the forelimbs of Mkx knockout mice [from embryonic day (E) 18.5 to postnatal day (P) 28 weeks] by using knocked-in Venus signals, Masson trichrome staining, and hematoxylin and eosin (H&E) staining.
RESULTS
We detected Venus signals in forelimb tendons, pulleys, and volar plates (VPs) in P21 mice. In-depth histological analysis showed that compared to the wild-type mice, the Mkx knockout mice showed significant hypoplasia in the flexor digitorum profundus tendons from E18.5. The VPs and pulleys appeared normal until P0; however, by P14, they became increasingly thicker in Mkx-null mice compared to wild-type mice. The fiber alignment was particularly disrupted in VPs of Mkx-null mice.
CONCLUSIONS
These results suggest that Mkx is an important regulator of the differentiation of VPs and pulleys, as well as of tendon differentiation.
背景
莫霍克（Mkx）是一种含同源结构域的转录因子，在各种中胚层衍生组织中表达，尤其是在发育中的肌腱中。在本研究中，我们调查了Mkx在前肢中的具体表达模式和功能。
方法
我们通过使用敲入的维纳斯信号、马松三色染色和苏木精-伊红（H&E）染色，分析了Mkx基因敲除小鼠的前肢（从胚胎第18.5天到出生后第28周）。
结果
我们在出生后21天小鼠的前肢肌腱、滑车和掌板（VP）中检测到维纳斯信号。深入的组织学分析表明，与野生型小鼠相比，Mkx基因敲除小鼠从胚胎第18.5天起，指深屈肌腱明显发育不全。掌板和滑车在出生后第0天看起来正常；然而，到出生后第14天，与野生型小鼠相比，Mkx基因敲除小鼠的掌板和滑车变得越来越厚。Mkx基因敲除小鼠掌板中的纤维排列尤其紊乱。
结论
这些结果表明，Mkx是掌板和滑车分化以及肌腱分化的重要调节因子。
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