Department of Plant Breeding and Biometry, Cornell University, 14853, Ithaca, N.Y., USA.
Theor Appl Genet. 1996 May;92(6):709-18. doi: 10.1007/BF00226093.
Some accessions of Lycopersicon pennellii, a wild relative of the tomato Lycopersicon esculentum, are resistant to a number of important pests of cultivated tomato due to the accumulation of acylsugars, which constitute 90% of the exudate of type-IV trichomes in L. pennellii LA716. An interspecific F2 population, created by the cross L. esculentum x L. pennellii LA 716, was surveyed for acylsugar accumulation and subjected to RFLP/QTL analysis to determine the genomic regions associated with the accumulation of acylglucoses, acylsucroses, and total acylsugars, as well as with acylglucoses as a percentage of total acylsugars (mole percent acylglucoses). Data were analyzed using MAPMAKER/QTL with and without a log10 transformation. A threshold value of 2.4 (default value for MAPMAKER/QTL) was used, as well as 95% empirically derived threshold values. Five genomic regions, two on chromosome 2 and one each on chromosomes 3, 4 and 11, were detected as being associated with one or more aspects of acylsugar production. The L. esculentum allele is partially dominant to the L. pennellii allele in the regions on chromosomes 2 and 11, but the L. pennellii allele is dominant in the region on chromosome 3. Throughout this study, we report the comparative effects of analytical methodology on the identification of acylsugar QTLs. Similarities between our results and published results for the genus Solanum are also discussed.
一些野生番茄的亲缘种——莱菔硫烷（Lycopersicon pennellii）的品系对许多重要的栽培番茄害虫具有抗性，这是由于在 IV 型毛状体的分泌物中，酰基糖的积累占 90%。通过番茄 L. esculentum x L. pennellii LA 716 的杂交，创建了一个种间 F2 群体，该群体被调查了酰基糖的积累情况，并进行了 RFLP/QTL 分析，以确定与酰基葡萄糖、酰基蔗糖和总酰基糖积累以及酰基葡萄糖占总酰基糖的比例（摩尔百分比酰基葡萄糖）相关的基因组区域。数据使用 MAPMAKER/QTL 进行了分析，包括和不包括对数转换。使用了 2.4 的阈值（MAPMAKER/QTL 的默认值），以及 95%的经验衍生阈值。检测到五个与酰基糖生产的一个或多个方面相关的基因组区域，两个在染色体 2 上，一个在染色体 3、4 和 11 上。在染色体 2 和 11 上，L. esculentum 等位基因对 L. pennellii 等位基因部分显性，但在染色体 3 上，L. pennellii 等位基因是显性的。在整个研究中，我们报告了分析方法对酰基糖 QTL 识别的比较影响。我们的结果与茄属 Solanum 发表的结果也进行了讨论。
