Department of Agronomy, Korea University, 136-701, Seoul, Korea.
Theor Appl Genet. 1996 May;92(6):719-25. doi: 10.1007/BF00226094.
RAPD (random amplified polymorphic DNA) markers generated by 15 arbitrary decamers were used to determine the frequency of DNA polymorphism in 39 watermelon [Citrullus lanantus (Thunb.) Mansf.] germplasms. Of the 15 primers tested, all except 1 (primer 275) directed the amplification of polymorphic products. A total of 162 amplification products were generated across all 39 genotypes. Among the 162 fragments, 35 (21%) appeared to be reliable polymorphic markers. The mean value by marker difference in this comparison was 0.24, and the highest, 0.69. Eight RAPD markers could be utilized in the unique variety discrimination 8 watermelon genotypes. From the phenograms constructed by UPGMA based on the comparison of RAPD markers, four clusters were resolved. Each group was also characterized and identified with morphological and genetic characteristics for each genotype. The free sugars of the edible parts of watermelons were analyzed by HPLC (high-performance liquid chromatography). Results from the phylogenetic analysis of band sharing data were consistent with sweetness as measured by HPLC. In conclusion, RAPD assays can be used for providing alternative markers for identifying genotypes and quantitative characteristics in watermelon.
RAPD（随机扩增多态性 DNA）标记由 15 个任意的十聚体产生，用于确定 39 个西瓜 [Citrullus lanantus（Thunb.）Mansf.] 种质的 DNA 多态性频率。在所测试的 15 个引物中，除 1 个（引物 275）外，所有引物都指导了多态性产物的扩增。总共在所有 39 个基因型中产生了 162 个扩增产物。在这 162 个片段中，有 35 个（21%）似乎是可靠的多态性标记。此比较中标记差异的平均值为 0.24，最高值为 0.69。8 个 RAPD 标记可用于独特品种鉴别 8 个西瓜基因型。基于 RAPD 标记比较构建的 UPGMA 聚类图，解析出 4 个聚类。还对每个组进行了特征描述和鉴定，以确定每个基因型的形态学和遗传学特征。通过 HPLC（高效液相色谱）分析西瓜可食用部分的游离糖。基于带共享数据的系统发育分析结果与 HPLC 测量的甜度一致。总之，RAPD 分析可用于提供替代标记，用于鉴定基因型和西瓜的定量特征。
