Plant Molecular Genetics Laboratory, Department of Plant and Soil Science and Institute for Biotechnology, Texas Tech University, 79409-2122, Lubbock, Texas, USA.
Theor Appl Genet. 1994 Jan;87(6):668-72. doi: 10.1007/BF00222891.
Genetic variation of nine upland and four lowland rice cultivars (Oryza sativa L.) was investigated at the DNA level using the randomly amplified polymorphic DNA (RAPD) method via the polymerase chain reaction (PCR). Forty-two random primers were used to amplify DNA segments and 260 PCR products were obtained. The results of agarosegel electrophoretic analysis of these PCR products indicated that 208 (80%) were polymorphic. All 42 primers used in this experiment were amplified and typically generated one-to-four major bands. Only two primers showed no polymorphisms. In general, a higher level of polymorphism was found between japonica and indica subspecies while fewer polymorphisms were found between upland and lowland cultivars within the indica subspecies. A dendrogram that shows the genetic distances of 13 rice cultivars was constructed based on their DNA polymorphisms. Classification of rice cultivars based on the results from the RAPD analysis was identical to the previous classification based on isozyme analysis. This study demonstrated that RAPD analysis is a useful tool in determining the genetic relationships among rice cultivars.
利用聚合酶链反应（PCR）的随机扩增多态性 DNA（RAPD）方法，在 DNA 水平上研究了 9 个旱地和 4 个水田水稻品种（Oryza sativa L.）的遗传变异。使用 42 个随机引物扩增 DNA 片段，得到 260 个 PCR 产物。琼脂糖凝胶电泳分析这些 PCR 产物的结果表明，208 个（80%）具有多态性。本实验中使用的 42 个引物均得到扩增，通常产生 1-4 条主要带。只有两个引物没有表现出多态性。一般来说，籼稻和粳稻亚种之间的多态性水平较高，而在籼稻亚种内，旱地和水田品种之间的多态性较少。根据 DNA 多态性构建了 13 个水稻品种遗传距离的聚类图。基于 RAPD 分析结果对水稻品种进行的分类与基于同工酶分析的先前分类相同。这项研究表明，RAPD 分析是确定水稻品种间遗传关系的有用工具。
