携带外源易位的小麦花药培养反应的遗传分析。

Genetic analysis of anther culture response in wheat carrying alien translocations.

机构信息

Laboratory of Genetics and Cytology, Agricultural Research Institute for South-East Regions, Tulaikov St., 7, 410020, Saratov, Russia.

出版信息

Theor Appl Genet. 1996 May;92(6):782-5. doi: 10.1007/BF00226102.

DOI:10.1007/BF00226102

PMID:24166404

Abstract

A bread wheat cultivar, 'Saratovskaya 29', (S29), its nearly isogenic lines carrying alien translocations [Lr9 from Aegilops umbellulata (Eg29) and (Lr19) from Agropyron elongatum (Ps29)] and two F1 hybrids between three nearly isogenic lines of S29 that differed by the Lr19+Rht1,Pro1+Pro2 and Ppd1+Ppd2 gene complexes, namely the S29 (Lr19+Rht1)/S29 (Ppd1+Ppd2) F1 and the S29 (Pro1+Pro2)/S29 (Lr19+Rht1) F1 were studied for their culture response with the following results. (1) Translocations with Lr9 and Lr19 decreased embryo frequency and green plant regeneration. (2) Both F1 hybrids showed a decrease in embryo frequency. One of the F1 hybrids, S29 (Lr19+Rht1)/S29 (Ppd1+Ppd2) showed a decrease, with respect to S29 for green plant regeneration; the other F1 S29 (Pro1+Pro2)/S29 (Lr19+Rht1), equalled S29 for green plant regeneration. (3) The gene complex of the F1 hybrid S29 (Pro1+Pro2)/S29 (Lr19+Rht1) was better than that of the F1 hybrid S29 (Lr19+Rht1)/S29 (Ppd1+Ppd2) for embryo induction and green plant regeneration. This effect was possibly induced by interactions between the Pro1+Pro2 and Lr19+Rht1 genes or was the result of direct actions of the Pro1+Pro2 genes.

摘要

一种面包小麦品种“萨拉托夫 29 号”（S29），及其携带异源易位的近等基因系[来自 Aegilops umbellulata 的 Lr9（Eg29）和来自 Agropyron elongatum 的 Lr19（Ps29）]，以及三个 S29 的近等基因系之间的两个 F1 杂种，它们在 Lr19+Rht1、Pro1+Pro2 和 Ppd1+Ppd2 基因复合物上存在差异，即 S29（Lr19+Rht1）/S29（Ppd1+Ppd2）F1 和 S29（Pro1+Pro2）/S29（Lr19+Rht1）F1，研究了它们的培养反应，结果如下。（1）携带 Lr9 和 Lr19 的易位降低了胚胎频率和绿苗再生率。（2）两个 F1 杂种均表现出胚胎频率降低。其中一个 F1 杂种 S29（Lr19+Rht1）/S29（Ppd1+Ppd2）的绿苗再生率相对于 S29 降低，而另一个 F1 杂种 S29（Pro1+Pro2）/S29（Lr19+Rht1）的绿苗再生率与 S29 相当。（3）F1 杂种 S29（Pro1+Pro2）/S29（Lr19+Rht1）的基因复合物在胚胎诱导和绿苗再生方面优于 F1 杂种 S29（Lr19+Rht1）/S29（Ppd1+Ppd2）。这种效应可能是由 Pro1+Pro2 和 Lr19+Rht1 基因之间的相互作用引起的，也可能是 Pro1+Pro2 基因的直接作用的结果。