Claus R, Schulze H A, Schulz U
Folia Haematol Int Mag Klin Morphol Blutforsch. 1985;112(5):683-8.
Side effects of both hypoosmotic and ammonium chloride-mediated hemolysis were compared looking for cellular integrity of human peripheral blood mononuclear cells. Recovery and viability of mononuclear cells significantly declined only following water treatment. Cell loss by lysis of non-erythrocyte cells (monitored by 51Cr release) preferentially occurred in the lymphocyte population resulting in a relative enrichment of monocytes (identified by peroxidase and esterase staining as well as phagocytosis of fluorescent latex particles). Consequences of this shifted monocyte/lymphocyte ratio for immunological tests are obvious. Necessity of red cell lysis and disadvantages referred especially to NH4Cl-induced white cell functional losses are outlined.
比较了低渗和氯化铵介导的溶血的副作用，以寻找人外周血单核细胞的细胞完整性。仅在水处理后，单核细胞的回收率和活力才显著下降。非红细胞细胞裂解导致的细胞损失（通过51Cr释放监测）优先发生在淋巴细胞群体中，导致单核细胞相对富集（通过过氧化物酶和酯酶染色以及荧光乳胶颗粒的吞噬作用鉴定）。这种单核细胞/淋巴细胞比例变化对免疫测试的影响是显而易见的。概述了红细胞裂解的必要性以及特别是氯化铵诱导的白细胞功能损失的缺点。
