人单核细胞对硝基蓝四氮唑还原的激活作用以及淋巴细胞对有丝分裂原反应的抑制作用。
Activation of human monocytes for nitroblue tetrazolium reduction and the suppression of lymphocyte response to mitogens.
作者信息
Zembala M, Lemmel E M, Uracz W
出版信息
Clin Exp Immunol. 1980 Aug;41(2):309-16.
Human peripheral blood mononuclear cells freed from polymorphs reduce nitroblue tetrazolium (NBT). This reduction is due to monocytes, i.e. adherent, phagocytic, esterase-positive cells with Fc receptors. Monocytes allowed to phagocytose zymosan show increased NBT reduction which under optimal conditions is 12.2 +/- 2.4 x 10(-9) mol . hr-1 . 10(-6) monocytes. Monocytes which have phagocytosed zymosan depress the mitogen response of human lymphocytes to PHA. This effect of 'activated' monocytes is due to a soluble inhibitory mediator which appears in the supernatant after culture for 24 hr. Its appearance requires protein synthesis. It is suggested that NBT reduction of peripheral blood mononuclear cells can be used as a test for the state of monocyte activation in disease. The possibility that activated monocytes may depress blast transformation in vitro in disease states is discussed.
去除多形核白细胞的人外周血单核细胞可使硝基蓝四氮唑（NBT）还原。这种还原是由单核细胞引起的，即具有Fc受体的贴壁、吞噬、酯酶阳性细胞。允许单核细胞吞噬酵母聚糖后，NBT还原增加，在最佳条件下为12.2±2.4×10⁻⁹摩尔·小时⁻¹·10⁻⁶个单核细胞。吞噬了酵母聚糖的单核细胞会抑制人淋巴细胞对PHA的有丝分裂反应。“活化”单核细胞的这种作用是由于一种可溶性抑制介质，该介质在培养24小时后出现在上清液中。它的出现需要蛋白质合成。有人提出，外周血单核细胞的NBT还原可作为疾病中单核细胞活化状态的一种检测方法。文中还讨论了活化单核细胞在疾病状态下可能在体外抑制母细胞转化的可能性。
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