USDA-ARS, Vegetable Crops Unit, Department of Horticulture, University of Wisconsin, 1575 Linden Dr., 53706, Madison, WI, USA.
Theor Appl Genet. 1996 May;92(7):865-72. doi: 10.1007/BF00221899.
Twenty-one polymorphic and 17 monomorphic cucumber (Cucumis sativus L.) isozyme loci were identified in 15 enzyme systems. Seven of the polymorphic loci (Ak-2, Ak-3, Fdp-1, Fdp-2, Mpi-1, Pep-gl, and Skdh) had not been described previously. Segregation in F2 and BC families for isozyme and morphological loci demonstrated agreement with the expected 1∶2∶1 and 1∶1 segregation ratio (P<0.01). Nine morphological markers were found to be linked to isozyme loci and were integrated to form a map containing four linkage groups spanning 584 cM with a mean linkage distance of approximately 19 cM. Linkage groups (A to D) contain the following loci in genetic order: A psl, Pep-la, B, Per, dm, Pgm, Mpi-1, Idh, Ar, Fdp-1, Ak-2, Pgd-1, Mpi-2 and gl; B lh, Mdh-2, Pep-gl, Pgd-2, Fdp-2, Ccu, Mdh-3, Ak-3, ll, de, F and Mdh-1, and Gr; C cor, Gpi, and Skdh; D Tu and ss. This study detected four new linkages between morphological markers (dm-psl, de-ll, ll-F, and de-F) and confirmed previously reported linkages, dm-Ar and Tu-ss. The isozyme/morphological map constructed in this study led to a more comprehensive understanding of the genetic relationships between several economically important traits.
在 15 种酶系统中，鉴定出 21 个多态性和 17 个单态性黄瓜（Cucumis sativus L.）同工酶位点。其中 7 个多态性位点（Ak-2、Ak-3、Fdp-1、Fdp-2、Mpi-1、Pep-gl 和 Skdh）以前尚未描述过。同工酶和形态学位点在 F2 和 BC 群体中的分离与预期的 1∶2∶1 和 1∶1 分离比例一致（P<0.01）。发现 9 个形态学标记与同工酶位点连锁，并整合形成一个图谱，包含四个连锁群，跨越 584cM，平均连锁距离约为 19cM。连锁群（A 至 D）按遗传顺序包含以下位点：A psl、Pep-la、B、Per、dm、Pgm、Mpi-1、Idh、Ar、Fdp-1、Ak-2、Pgd-1、Mpi-2 和 gl；B lh、Mdh-2、Pep-gl、Pgd-2、Fdp-2、Ccu、Mdh-3、Ak-3、ll、de、F 和 Mdh-1，以及 Gr；C cor、Gpi 和 Skdh；D Tu 和 ss。本研究检测到形态标记（dm-psl、de-ll、ll-F 和 de-F）之间的四个新连锁，并证实了先前报道的 dm-Ar 和 Tu-ss 连锁。本研究构建的同工酶/形态学图谱使我们对几个经济重要性状之间的遗传关系有了更全面的了解。
