Van Laere O, De Wael L, De Mey J
Histochemistry. 1985;83(5):397-9. doi: 10.1007/BF00509198.
For the identification of the plant pathogenic bacterium Erwinia amylovora, the immuno gold staining (IGS) and immuno gold silver staining (IGSS) techniques are tested. The IGS and IGSS methods are at least as sensitive an indirect immunofluorescence and require less primary antiserum. Moreover they have the advantage that the preparations can be conserved permanently and unchanged. The preparation of the IGS can be observed with transmitted light or--with considerable better result--using epipolarization microscopy. The IGSS method deserves special attention because of its high contrast in normal brigth field microscopy with transmitted light.
为鉴定植物病原菌梨火疫病菌，对免疫金染色（IGS）和免疫金银染色（IGSS）技术进行了测试。IGS和IGSS方法至少与间接免疫荧光一样灵敏，且所需的一抗血清较少。此外，它们的优点是制备物可以永久保存且不变。IGS的制备物可以用透射光观察，或者——效果更好的是——使用落射偏光显微镜观察。由于IGSS方法在普通明场显微镜透射光下具有高对比度，因此值得特别关注。
