Magar R, Larochelle R
Laboratoire d'Hygiène Vétérinaire et Alimentaire, Agriculture Canada, St-Hyacinthe, PQ.
J Vet Diagn Invest. 1992 Jan;4(1):3-7. doi: 10.1177/104063879200400102.
Immunogold silver staining (IGSS) was applied for the detection of porcine group A rotavirus in formalin-fixed paraffin-embedded tissue sections of small intestine. Prior to the application of IGSS, the reactivity of protein A-gold as a marker was tested with group-specific antiserum in immunogold electron microscopy. Immune aggregates were intensely and specifically labeled with the gold complex. Application of IGSS to tissue sections resulted in specific dark staining of villous enterocytes infected by group A rotavirus. This method also proved effective for the detection of rotaviral antigen in infected cultured cells. The IGSS method may be suitable for routine diagnostic detection of rotaviral infections and may have application for detection of other viral pathogens of veterinary importance.
免疫金-银染色法（IGSS）用于检测福尔马林固定、石蜡包埋的小肠组织切片中的猪A组轮状病毒。在应用IGSS之前，在免疫金电子显微镜下用群特异性抗血清测试了作为标记物的蛋白A-金的反应性。免疫聚集体被金复合物强烈且特异性地标记。将IGSS应用于组织切片导致被A组轮状病毒感染的绒毛肠上皮细胞出现特异性深色染色。该方法也被证明对检测感染的培养细胞中的轮状病毒抗原有效。IGSS方法可能适用于轮状病毒感染的常规诊断检测，并且可能可用于检测其他具有兽医重要性的病毒病原体。
