Gaitas Angelo, Malhotra Ricky, Pienta Kenneth
PicoCal, Inc., 333 Parkland Plaza, Ann Arbor, Michigan 48103, USA ; Electronic Instrumentation Laboratory, TU Delft, Mekelweg 4, 2628CD Delft, The Netherlands.
Appl Phys Lett. 2013 Sep 16;103(12):123702. doi: 10.1063/1.4821946. Epub 2013 Sep 19.
In the present study we engineered a micro-machined polyimide cantilever with an embedded sensing element to investigate cellular adhesion, in terms of its relative ability to stick to a cross-linker, 3,3'-dithiobis[sulfosuccinimidylpropionate], coated on the cantilever surface. To achieve this objective, we investigated adhesive properties of three human prostate cancer cell lines, namely, a bone metastasis derived human prostate cancer cell line (PC3), a brain metastasis derived human prostate cancer cell line (DU145), and a subclone of PC3 (PC3-EMT14). We found that PC3-EMT14, which displays a mesenchymal phenotype, has the least adhesion compared to PC3 and DU145, which exhibit an epithelial phenotype.
在本研究中，我们设计了一种带有嵌入式传感元件的微机械加工聚酰亚胺悬臂梁，以研究细胞粘附情况，具体是通过细胞粘附到涂覆在悬臂梁表面的交联剂3,3'-二硫代双[磺基琥珀酰亚胺丙酸酯]上的相对能力来进行研究。为实现这一目标，我们研究了三种人前列腺癌细胞系的粘附特性，即骨转移来源的人前列腺癌细胞系（PC3）、脑转移来源的人前列腺癌细胞系（DU145）以及PC3的一个亚克隆（PC3-EMT14）。我们发现，呈现间充质表型的PC3-EMT14与表现上皮表型的PC3和DU145相比，其粘附性最低。
