Jahangir Tamanna, Safhi Mohammed M, Sultana Sarwat, Ahmad Sayeed
Department of Pharmacology, College of Pharmacy, Jazan University, Saudi Arabia.
Interdiscip Toxicol. 2013 Mar;6(1):47-54. doi: 10.2478/intox-2013-0009.
Evidence from epidemiological, experimental and clinical trial data indicates that a plant based diet can reduce the risk of chronic diseases and reduces toxic effects. In the present study, we report the antioxidant and anticlastogenic activity of Pluchea lanceolata (PL), an important medicinal plant, in both in vitro and in vivo model. Benzo(a)pyrene (B(a)P) administration leads to depletion of renal glutathione and its metabolizing enzymes. Pretreatment with PL (100 and 200 mg /kg b.wt) restored renal glutathione content and its dependent enzymes significantly (p<0.001) with simultaneous increase in catalase(CAT), quinone reductase(QR) in mouse kidney. Prophylactic administration of PL prior to B (a) P administration significantly decreased the malondialdehyde(MDA), H2O2 and xanthineoxidase (XO) levels at a significance of p<0.001, at both the doses. PL extract pretreated groups showed marked inhibition in B(a)P induced micronuclei formation in mouse bone marrow cells with simultaneous restoration of DNA integrity, viz. alkaline unwinding assay and DNA damage shown by gel-electrophoresis. HPTLC confirms the presence of quercetin in plant extract which could be responsible for PL protecting efficacy. In conclusion, the present findings strongly support the antioxidant efficacy of PL, possibly by modulation of antioxidant armory.
来自流行病学、实验和临床试验数据的证据表明，以植物为基础的饮食可以降低慢性病风险并减少毒性作用。在本研究中，我们报告了重要药用植物披针叶蒲葵（PL）在体外和体内模型中的抗氧化和抗致突变活性。给予苯并(a)芘（B(a)P）会导致肾脏谷胱甘肽及其代谢酶的消耗。用PL（100和200毫克/千克体重）预处理可显著恢复肾脏谷胱甘肽含量及其相关酶（p<0.001），同时小鼠肾脏中的过氧化氢酶（CAT）、醌还原酶（QR）增加。在给予B(a)P之前预防性给予PL，在两个剂量下均显著降低了丙二醛（MDA）、H2O2和黄嘌呤氧化酶（XO）水平，p<0.001。PL提取物预处理组在小鼠骨髓细胞中对B(a)P诱导的微核形成有明显抑制作用，同时恢复了DNA完整性，即碱性解旋试验和凝胶电泳显示的DNA损伤。高效薄层层析证实植物提取物中存在槲皮素，这可能是PL具有保护功效的原因。总之，本研究结果有力地支持了PL的抗氧化功效，可能是通过调节抗氧化物质实现的。
