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通过实时 PCR 检测饲喂和不饲喂银合欢(Leucaena leucocephala)杂交种的山羊瘤胃厌氧纤维分解真菌种群。

Anaerobic cellulolytic rumen fungal populations in goats fed with and without Leucaena leucocephala hybrid, as determined by real-time PCR.

机构信息

Institute of Bioscience, Universiti Putra Malaysia, Selangor, 43400, Malaysia.

出版信息

J Microbiol. 2013 Oct;51(5):700-3. doi: 10.1007/s12275-013-2540-z. Epub 2013 Oct 31.

DOI:10.1007/s12275-013-2540-z
PMID:24173648
Abstract

The effect of Leucaena leucocephala hybrid-Bahru (LLB), which contains a high concentration of condensed tannins, on cellulolytic rumen fungal population in goats was investigated using real-time PCR. The fungal population in goats fed LLB was inhibited during the first 10 days of feeding, but after 15 days of feeding, there was a tremendous increase of fungal population (157.0 μg/ml), which was about fourfold more than that in control goats (39.7 μg/ml). However, after this period, the fungal population decreased continuously, and at 30 days of feeding, the fungal population (50.6 μg/ml) was not significantly different from that in control goats (55.4 μg/ml).

摘要

采用实时 PCR 技术研究了富含单宁的杂交银合欢(Leucaena leucocephala hybrid-Bahru,LLB)对山羊瘤胃纤维素分解真菌种群的影响。在饲喂 LLB 的前 10 天,山羊的真菌种群受到抑制,但在 15 天后,真菌种群(157.0μg/ml)急剧增加,是对照组山羊(39.7μg/ml)的四倍多。然而,在此期间后,真菌种群持续下降,在 30 天的喂养期,真菌种群(50.6μg/ml)与对照组山羊(55.4μg/ml)没有显著差异。

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Phylogenetic analysis of 16S rRNA gene sequences reveals rumen bacterial diversity in Yaks (Bos grunniens).基于16S rRNA基因序列的系统发育分析揭示了牦牛(Bos grunniens)瘤胃细菌的多样性。
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