Plant Biotechnology Institute, National Research Council of Canada, 110 Gymnasium Place, S7N OW9, Saskatoon, Saskatchewan, Canada.
Theor Appl Genet. 1995 Apr;90(5):607-10. doi: 10.1007/BF00222122.
Restriction fragment analysis was used to study the inheritance of chloroplast DNA (cpDNA) in F1 progeny from crosses between Lens culinaris ssp. orientalis and L. culinaris ssp. culinaris. Twenty-five combinations of 11 restriction enzymes and three heterologous probes from Petunia hybrida cpDNA were used to screen six accessions of L.c. culinaris and one accession of L. c. orientalis for restriction fragment length polymorphisms (RFLPs). No variation in cpDNA was observed within the subspecies L. c. culinaris, but the L. c. orientalis accession was unambiguously distinguished from all six L. c. culinaris accessions by two RFLPs. Of ten F1 progeny from L. c. orientalis x L. c. culinaris crosses, nine had only maternal cpDNA restriction fragments but one F1 plant inherited cpDNA fragments from both parents. Nuclear DNA inheritance was biparental in all ten F1 progeny.
采用限制片段分析的方法,对东方鸭茅(Lens culinaris ssp.orientalis)和普通鸭茅(L.culinaris ssp.culinaris)杂交后代 F1 代叶绿体 DNA(cpDNA)的遗传进行了研究。使用来自矮牵牛(Petunia hybrida)cpDNA 的 11 种限制酶和 3 种异源探针的 25 种组合,对 6 个普通鸭茅品系和 1 个东方鸭茅品系进行了筛选,以寻找限制片段长度多态性(RFLP)。在普通鸭茅亚种内未观察到 cpDNA 的变化,但通过 2 个 RFLP,可将东方鸭茅品系与所有 6 个普通鸭茅品系明确区分开来。在东方鸭茅(L.c.orientalis)×普通鸭茅(L.c.culinaris)杂交的 10 个 F1 代后代中,有 9 个只具有母本 cpDNA 限制片段,但有一个 F1 植物从双亲遗传了 cpDNA 片段。在所有 10 个 F1 代后代中,核 DNA 的遗传都是双亲的。