Plant Biotechnology Institute, National Research Council of Canada, 110 Gymnasium Place, S7N 0W9, Saskatoon, Saskatchewan, Canada.
Theor Appl Genet. 1994 Oct;89(2-3):206-10. doi: 10.1007/BF00225143.
Restriction fragment analysis was used to examine the inheritance of lentil mitochondrial DNA (mtDNA) in F1 and F5 progeny from intrasubspecific (Lens culinaris ssp. culinaris) crosses and in F1 progeny from intersubspecific (Lens culinaris ssp. orientalis x L. culinaris ssp. culinaris) crosses. Southern blots of digested parental and progeny DNA were hybridized to heterologous maize mtDNA probes specific to coxI and atp6 genes. Two restriction fragment polymorphisms separated L.c. ssp. culinaris 'Laird' and 'Eston' from L.c. ssp. culinaris 'ILL5588', and one restriction fragment polymorphism distinguished L.c. ssp. culinaris 'Laird' and 'Eston' from L.c. ssp. orientalis 'LO4'. Twelve of 13 f1 progeny and all F5 progeny from the intrasubspecific crosses, and all F1 progeny from intersubspecific crosses had only maternal mtDNA restriction fragments. One f1 plant from an 'Eston' x 'ILL5588' cross inherited mtDNA fragments from both parents. Nuclear DNA inheritance was biparental in all F1 progeny.
利用限制性片段分析研究了亚种内（菜豆 Lens culinaris ssp. culinaris）杂交的 F1 和 F5 后代以及亚种间（菜豆 Lens culinaris ssp. orientalis x Lens culinaris ssp. culinaris）杂交的 F1 后代中莱豆线粒体 DNA（mtDNA）的遗传情况。消化后的亲本和后代 DNA 的 Southern 印迹与异源玉米 mtDNA 探针coxI 和 atp6 基因杂交。两种限制性片段多态性将 L.c. ssp. culinaris 'Laird' 和 'Eston' 与 L.c. ssp. culinaris 'ILL5588' 区分开来，一种限制性片段多态性将 L.c. ssp. culinaris 'Laird' 和 'Eston' 与 L.c. ssp. orientalis 'LO4' 区分开来。13 个亚种内杂交的 F1 后代中的 12 个和所有 F5 后代以及亚种间杂交的所有 F1 后代都只有母系 mtDNA 限制片段。来自 'Eston' x 'ILL5588' 杂交的一个 F1 植物从父母双方遗传了 mtDNA 片段。所有 F1 后代的核 DNA 遗传都是双亲的。
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