Musengi A, Khan N, Le Roes-Hill M, Pletschke B I, Burton S G
Biocatalysis and Technical Biology Research Group, Cape Peninsula University of Technology, Bellville, South Africa.
J Appl Microbiol. 2014 Mar;116(3):554-62. doi: 10.1111/jam.12380. Epub 2013 Nov 19.
To optimize peroxidase production by Streptomyces sp. strain BSII#1, up to 3 l culture volumes.
Peroxidase production by Streptomyces sp. strain BSII#1 was optimized in terms of production temperature and pH and the use of lignin-based model chemical inducers. The highest peroxidase activity (1·30 ± 0·04 U ml(-1) ) in 10 ml culture volume was achieved in a complex production medium (pH 8·0) at 37°C in the presence of 0·1 mmol l(-1) veratryl alcohol, which was greater than those reported previously. Scale-up to 100 and 400 ml culture volumes resulted in decreased peroxidase production (0·53 ± 0·10 and 0·26 ± 0·08 U ml(-1) , respectively). However, increased aeration improved peroxidase production with the highest production achieved using an airlift bioreactor (4·76 ± 0·46 U ml(-1) in 3 l culture volume).
Veratryl alcohol (0·1 mmol l(-1) ) is an effective inducer of peroxidase production by Streptomyces sp. strain BSII#1. However, improved aeration increased peroxidase production in larger volumes without the use of an inducer, surpassing induced yields in an optimized small-scale process.
Only a limited number of reports in literature have focused on the up-scaling of bacterial peroxidase production. There remains opportunity for feasible large-scale production of bacterial peroxidases with potentially novel biocatalytic properties.
优化链霉菌属菌株BSII#1的过氧化物酶产量，培养体积可达3升。
从生产温度、pH值以及使用基于木质素的模型化学诱导剂方面对链霉菌属菌株BSII#1的过氧化物酶产量进行了优化。在10毫升培养体积中，于37°C的复合生产培养基（pH 8.0）中，在存在0.1毫摩尔/升藜芦醇的情况下，实现了最高过氧化物酶活性（1.30±0.04单位/毫升），这高于先前报道的数值。扩大到100毫升和400毫升培养体积导致过氧化物酶产量下降（分别为0.53±0.10和0.26±0.08单位/毫升）。然而，增加通气量可提高过氧化物酶产量，使用气升式生物反应器实现了最高产量（3升培养体积中为4.76±0.46单位/毫升）。
藜芦醇（0.1毫摩尔/升）是链霉菌属菌株BSII#1过氧化物酶生产的有效诱导剂。然而，改善通气量可在不使用诱导剂的情况下提高较大体积培养中的过氧化物酶产量，超过优化的小规模工艺中的诱导产量。
文献中仅有有限数量的报告关注细菌过氧化物酶生产的放大。对于具有潜在新型生物催化特性的细菌过氧化物酶的可行大规模生产仍存在机会。
