E. H. Graham Centre for Agricultural Innovation, Charles Sturt University, Locked Bag 588, Wagga, NSW 2678, Australia; School of Agricultural and Wine Sciences, Charles Sturt University, Locked Bag 588, Wagga, NSW 2678, Australia; Department of Human Nutritional Sciences, The Richardson Center for Functional Foods and Nutraceuticals, University of Manitoba, Winnipeg R3T 2N2, Canada.
Food Chem. 2014 Mar 1;146:500-6. doi: 10.1016/j.foodchem.2013.09.081. Epub 2013 Sep 25.
Antioxidant activities of canola protein hydrolysates (CPHs) and peptide fractions prepared using five proteases and ultrafiltration membranes (1, 3, 5, and 10kDa) were investigated. CPHs had similar and adequate quantities of essential amino acids. The effective concentration that scavenged 50% (EC50) of the ABTS(+) was greatest for the <1kDa pancreatin fraction at 10.1μg/ml. CPHs and peptide fractions scavenged DPPH(+) with most of the EC50 values being <1.0mg/ml. Scavenging of superoxide radical was generally weak, except for the <1kDa pepsin peptide fraction that had a value of 51%. All CPHs inhibited linoleic acid oxidation with greater efficiency observed for pepsin hydrolysates. The oxygen radical absorbance capacity of Alcalase, chymotrypsin and pepsin hydrolysates was found to be better than that of glutathione (GSH) (p<0.05). These results show that CPHs have the potential to be used as bioactive ingredients in the formulation of functional foods against oxidative stress.
研究了使用五种蛋白酶和超滤膜（1、3、5 和 10 kDa）制备的油菜蛋白水解物（CPHs）和肽级分的抗氧化活性。CPHs 具有相似且适量的必需氨基酸。ABTS(+) 的 50%清除有效浓度（EC50）最高的是 10.1μg/ml 的<1kDa 胰蛋白酶级分。CPHs 和肽级分清除 DPPH(+)，大多数 EC50 值均<1.0mg/ml。超氧自由基的清除作用通常较弱，除了<1kDa 胃蛋白酶肽级分的清除率为 51%。所有 CPHs 均抑制亚油酸氧化，胃蛋白酶水解物的抑制效率更高。发现 Alcalase、胰凝乳蛋白酶和胃蛋白酶水解物的氧自由基吸收能力优于谷胱甘肽（GSH）（p<0.05）。这些结果表明，CPHs 有可能作为功能性食品中抗氧化应激的生物活性成分。
