Arise Abimbola K, Alashi Adeola M, Nwachukwu Ifeanyi D, Malomo Sunday A, Aluko Rotimi E, Amonsou Eric O
Department of Biotechnology and Food Technology, Faculty of Applied Sciences, Durban University of Technology, PO Box 1334, Durban 4001, South Africa.
Department of Human Nutritional Sciences, University of Manitoba, Winnipeg, MB, R3T 2 N2, Canada.
J Sci Food Agric. 2017 Jul;97(9):2834-2841. doi: 10.1002/jsfa.8112. Epub 2016 Dec 21.
An increased rate of high blood pressure has led to critical human hypertensive conditions in most nations. In the present study, bambara protein hydrolysates (BPHs) obtained using three different proteases (alcalase, trypsin and pepsin) and their peptide fractions (molecular weight: 10, 5, 3 and 1 kDa) were investigated for antihypertensive and antioxidant activities.
Alcalase hydrolysate contained the highest amount of low molecular weight (LMW) peptides compared to pepsin and trypsin hydrolysates. LMW peptides fractions (<1 kDa) exhibited the highest inhibitory activity against angiotensin-converting enzyme (ACE) for all the enzymes hydrolysates. For renin inhibition, alcalase hydrolysate showed the highest inhibition at 59% compared to other hydrolysates and their corresponding membrane fractions. The antioxidant power of bambara protein hydrolysates and peptide fractions was evaluated through the inhibition of linoleic acid peroxidation and ABTS scavenging activity. Among the hydrolysates, alcalase exhibited the highest inhibition of linoleic acid oxidation. Furthermore, all BPHs were able to scavenge ABTS to a three-fold greater extent compared to the isolate.
BPH and LMW peptide fractions could potentially serve as useful ingredients in the formulation of functional foods and nutraceuticals against high blood pressure and oxidative stress. © 2016 Society of Chemical Industry.
在大多数国家，高血压发病率的上升已导致严重的人类高血压疾病。在本研究中，对使用三种不同蛋白酶（碱性蛋白酶、胰蛋白酶和胃蛋白酶）获得的班巴拉蛋白水解物（BPHs）及其肽组分（分子量分别为10、5、3和1 kDa）的降压和抗氧化活性进行了研究。
与胃蛋白酶和胰蛋白酶水解物相比，碱性蛋白酶水解物含有最高量的低分子量（LMW）肽。对于所有酶水解物，LMW肽组分（<1 kDa）对血管紧张素转换酶（ACE）表现出最高的抑制活性。对于肾素抑制，碱性蛋白酶水解物与其他水解物及其相应的膜组分相比，显示出最高的抑制率，为59%。通过抑制亚油酸过氧化和ABTS清除活性来评估班巴拉蛋白水解物和肽组分的抗氧化能力。在水解物中，碱性蛋白酶对亚油酸氧化的抑制作用最强。此外，与分离物相比，所有BPHs清除ABTS的能力提高了三倍。
BPH和LMW肽组分有可能作为功能性食品和营养保健品配方中的有用成分，用于对抗高血压和氧化应激。©2016化学工业协会。
