Department of Biochemistry and Applied Molecular Biology, University of Manchester Institute of Science and Technology, M60 1QD, Manchester.
Curr Genet. 1984 Sep;8(7):489-92. doi: 10.1007/BF00410434.
The nucleotide sequence of a 74 codon reading frame from the Aspergillus nidulans mitochondrial genome is presented. The derived amino acid sequence displays typical features of dicyclohexylcarbodiimide (DCCD) binding proteins and is 84% homologous with a mitochondrial reading frame that potentially encodes an ATPase subunit 9 polypeptide in Neurospora crassa. However, in A. nidulans, as in N. crassa, there is strong biochemical and genetic evidence that this subunit is in fact nuclearly-encoded. In both organisms the DCCD-binding protein found in the F0 complexes of mitochondria from actively-growing cultures is almost certainly the product of this nuclear gene, and definitely not that of the mitochondrial reading frame. The discovery of an intact open reading frame than can code for a DCCD-binding protein in the mitochondrial genome of a second species of filamentous fungus strenghthens the possibility that the presence of a mitochondrial version of this gene has some biological significance.
本文呈现了来自构巢曲霉线粒体基因组的 74 密码子阅读框的核苷酸序列。推导的氨基酸序列显示出二环己基碳二亚胺（DCCD）结合蛋白的典型特征，与潜在编码 Neurospora crassa 中 ATP 酶亚基 9 多肽的线粒体阅读框具有 84%的同源性。然而，在构巢曲霉中，与 Neurospora crassa 一样，有强有力的生化和遗传证据表明，该亚基实际上是核编码的。在这两种生物中，从活跃生长培养物的线粒体 F0 复合物中发现的 DCCD 结合蛋白几乎肯定是该核基因的产物，而不是线粒体阅读框的产物。在第二种丝状真菌的线粒体基因组中发现一个完整的可读框，能够编码 DCCD 结合蛋白，这增强了该基因的线粒体版本存在具有某种生物学意义的可能性。
