Ward M, Turner G
Mol Gen Genet. 1986 Nov;205(2):331-8. doi: 10.1007/BF00430447.
We have determined the nucleotide sequence of the Aspergillus nidulans nuclear gene oliC31, which encodes subunit 9 of mitochondrial ATP synthase. The open reading frame contains no introns and specifies a predicted protein of 143 amino acids comprising a pre-sequence of 62 residues and a mature protein of 81 residues. The amino acid homology with the equivalent Neurospora crassa protein is 50% for the pre-sequence and 80% for the mature protein. A comparison with this and other imported mitochondrial proteins has revealed conserved regions which may be important for transport or subsequent processing. Multiple transcription initiation and polyadenylation sites have been identified. The promoter region of the oliC31 gene is characterised by long pyrimidine-rich tracts preceding the transcription initiation sites.
我们已经确定了构巢曲霉核基因oliC31的核苷酸序列，该基因编码线粒体ATP合酶的亚基9。开放阅读框不含内含子，编码一个预测的由143个氨基酸组成的蛋白质，其中包括一个62个残基的前导序列和一个81个残基的成熟蛋白。与粗糙脉孢菌的等效蛋白相比，前导序列的氨基酸同源性为50%，成熟蛋白的氨基酸同源性为80%。与该蛋白及其他导入的线粒体蛋白进行比较后，发现了一些保守区域，这些区域可能对转运或后续加工很重要。已鉴定出多个转录起始和聚腺苷酸化位点。oliC31基因的启动子区域的特征是在转录起始位点之前有长的富含嘧啶的序列。
