RAPD 标记在油棕（Elaeis guineensis）遗传变异测定中的应用。

The utility of RAPD markers for the determination of genetic variation in oil palm (Elaeis guineensis).

机构信息

Genetics Department, National University of Malaysia, Selangor, Malaysia.

出版信息

Theor Appl Genet. 1994 Nov;89(6):713-8. doi: 10.1007/BF00223710.

Abstract

The genetic variation among different accessions of oil-palm germplasm collected from Africa was estimated using random primers and the polymerase chain reaction. The present study revealed high levels of genetic variation in these accessions. Electrophoresis of the amplification products indicated that nine out of 20 primers were able to generate polymorphic products ranging in length from 0.2 kb to 2.3 kb. No individual palm or population-specific products were observed. Greatest diversity was seen in Zaire population 5 and the least in Zaire population 2.

摘要

利用随机引物和聚合酶链反应（PCR）技术对来自非洲的油棕种质资源不同品系的遗传变异进行了估计。本研究揭示了这些品系具有高水平的遗传变异。扩增产物的电泳分析表明，20 个引物中有 9 个能够产生长度在 0.2kb 到 2.3kb 之间的多态性产物。没有观察到特定于个体或群体的产物。在扎伊尔种群 5 中观察到最大的多样性，而在扎伊尔种群 2 中观察到最小的多样性。