Chalmers K J, Waugh R, Sprent J I, Simons A J, Powell W
Scottish Crop Research Institute, Invergowrie, Dundee.
Heredity (Edinb). 1992 Nov;69 ( Pt 5):465-72. doi: 10.1038/hdy.1992.151.
Gliricidia sepium and G. maculata are multi-purpose leguminous trees native to Central America and Mexico. Research programmes have been initiated to define the native distribution of Gliricidia and sample the spectrum of genetic variation. To date, there has been little systematic assessment of genetic variability in multi-purpose tree species. Accurate estimates of diversity between- and within-populations are considered a prerequisite for the optimization of sampling and breeding strategies. We have used a PCR-based polymorphic assay procedure (RAPDs) to monitor genetic variability in Gliricidia. Extensive genetic variability was detected between species and the variability was partitioned into between- and within-population components. On average, most (60 per cent) of the variation occurs between G. sepium populations but oligonucleotide primers differed in their capacity to detect variability between and within populations. Population-specific genetic markers were identified. RAPDs provide a cost-effective method for the precise and routine evaluation of variability and may be used to identify areas of maximum diversity. The approaches outlined have general applicability to a range of organisms and are discussed in relation to the exploitation of multi-purpose tree species of the tropics.
紫穗槐和斑点紫穗槐是原产于中美洲和墨西哥的多用途豆科树木。已经启动了研究项目来确定紫穗槐的原生分布并对遗传变异谱进行采样。迄今为止，对多用途树种的遗传变异性几乎没有进行系统评估。准确估计种群间和种群内的多样性被认为是优化采样和育种策略的先决条件。我们使用基于PCR的多态性检测程序（随机扩增多态性DNA）来监测紫穗槐的遗传变异性。在物种间检测到了广泛的遗传变异性，并且这种变异性被分为种群间和种群内成分。平均而言，大部分（60%）变异发生在紫穗槐种群之间，但寡核苷酸引物在检测种群间和种群内变异性的能力上有所不同。鉴定出了种群特异性遗传标记。随机扩增多态性DNA为精确和常规评估变异性提供了一种经济有效的方法，可用于识别最大多样性区域。所概述的方法对一系列生物体具有普遍适用性，并结合热带多用途树种的开发进行了讨论。
