通过对成熟树木的顶芽和节段外植体进行离体培养，实现三种桑树（华桑、鲁桑和裂叶桑）的快速克隆繁殖。

Rapid clonal propagation of three mulberries,Morus cathayana HemsL,M. lhou Koiz. andM. serrata Roxb., through in vitro culture of apical shoot buds and nodal explants from mature trees.

作者信息

Pattnaik S K, Chand P K

机构信息

Plant Tissue and Cell Culture Facility, Post-Graduate Department of Botany, Utkal University, Bhubaneswar-751 004, Orissa, India.

出版信息

Plant Cell Rep. 1997 Apr;16(7):503-508. doi: 10.1007/BF01092774.

DOI:10.1007/BF01092774

PMID:30727640

Abstract

High-frequency bud break and multiple shoots were induced in apical shoot buds and nodal explants ofMorus cathayana, M. lhou andM. serrata on Murashige and Skoog (MS) medium containing 0.5-1.0 mg/l 6-benzylaminopurine (BAP). Addition of gibberellic acid (0.4 mg/l) along with BAP induced faster bud break both in apical shoot buds and nodal explants and also enhanced the frequency of bud break in all three species. Shoot culture initiation was greatly influenced by explant type, explant age and explanting season. The shoots were successfully rooted on half-strength MS medium containing a combination of indole-3-acetic acid, indole-3-butyric acid and indole-3-propionic acid, each at 1.0 mg/l. The plantlets were successfully acclimated and eventually established in soil.

摘要

在含有0.5 - 1.0毫克/升6 - 苄基腺嘌呤（BAP）的Murashige和Skoog（MS）培养基上，从华桑、鲁桑和鸡桑的顶芽和节外植体中诱导出高频芽萌发和多芽。赤霉素（0.4毫克/升）与BAP一起添加，能使顶芽和节外植体的芽萌发更快，并且提高了所有三个物种的芽萌发频率。芽培养的起始受到外植体类型、外植体年龄和取材季节的极大影响。将芽接种在含有1.0毫克/升吲哚 - 3 - 乙酸、吲哚 - 3 - 丁酸和吲哚 - 3 - 丙酸组合的1/2强度MS培养基上，成功诱导生根。试管苗成功驯化并最终移栽到土壤中。