Institut de Biotechnologie des plantes, URA CNRS D-1128, Université de Paris-Sud, F-91405, Orsay Cedex, France.
Plant Cell Rep. 1996 Sep;15(12):900-4. doi: 10.1007/BF00231584.
Callus induction, somatic embryogenesis and plant regeneration were obtained in two cultivars of Sorghum bicolor (L.) Moench. Transverse thin cell layers from roots/epicotyls of 15-day-old seedlings or of regenerated plantlets were used. Callus response depended on the genotype, the size of transverse thin cell layers, the level at which transverse thin cell layers were excised on the epicotyl, the composition of growth substances and the number of in vitro regeneration cycles undergone by the donor plant. Somatic embryos were differentiated under a defined dark/light sequence, from epidermised compact calluses (i.e having already differentiated an epidermis), obtained directly with dicamba or from friable callus initiated with kinetin and 2,4 dichlorophenoxyacetic acid. The importance of kinetin and dicamba on the induction of embryogenic potential is reported.
在两个甜高粱（高粱）品种中诱导愈伤组织、体细胞胚胎发生和植株再生。使用来自 15 天大的幼苗或再生苗的根/上胚轴的横向薄细胞层。愈伤组织反应取决于基因型、横向薄细胞层的大小、在上胚轴上切取横向薄细胞层的水平、生长物质的组成和供体植物经历的体外再生循环的数量。体细胞胚从已分化出表皮的表皮化致密愈伤组织（即已经分化出表皮），或从用激动素和 2,4-二氯苯氧乙酸起始的易碎愈伤组织中，在定义的黑暗/光照序列下分化。报道了激动素和二甲苯对诱导胚胎发生潜能的重要性。
