Department of Botany, Colorado State University, 80523, Ft. Collins, CO, USA.
Plant Cell Rep. 1986 Oct;5(5):349-51. doi: 10.1007/BF00268599.
Tissue culture methods were developed for the induction, maintenance, and regeneration of embryogenic callus in sweet sorghum (Sorghum bicolor) cultivars Keller, Rio, and Wray. No significant differences were observed in production of embryogenic callus in cultures established from developmentally immature or mature embryo explants cultured on LS medium with 2 mg/1 2,4-D plus 0.5 mg/1 kinetin. Prolific callus production did not occur until the third four-week culture period. Long-term maintenance of embryogenic callus was dependent upon the selective transfer of embryogenic callus, with other callus types discarded. High-frequency plant regeneration was achieved and quantified on a fresh weight basis of embryogenic callus.
组织培养方法被开发出来，用于诱导、维持和再生甜高粱（Sorghum bicolor）品种 Keller、Rio 和 Wray 的胚性愈伤组织。在 LS 培养基上培养的发育未成熟或成熟的胚外植体上，2 mg/1 2,4-D 加 0.5 mg/1 激动素，培养的胚性愈伤组织在产量上没有明显差异。直到第三个四周的培养期，才会产生大量的愈伤组织。胚性愈伤组织的长期维持取决于胚性愈伤组织的选择性转移，其他类型的愈伤组织被丢弃。通过对胚性愈伤组织的鲜重进行高频植物再生，实现了定量再生。
