Department of Plant and Microbial Sciences, University of Canterbury, Private Bag 4800, 1, Christchurch, New Zealand.
Plant Cell Rep. 1996 Sep;15(12):974-9. doi: 10.1007/BF00231599.
The simultaneous presence of 6-benzyladenine (BA) and sucrose in a Murashige and Skoog medium (SIM) during the initial stages of shoot initiation have been found to be obligatory for high-frequency shoot formation in the Capsicum annuum L. var. 'Sweet Banana' upper hypocotyl explants. The explants are determined for shoot formation following a minimum of 8 days of culture on SIM. Deprivation of exogenous sucrose from day 6 to day 20 of culture had no effect on the shoot forming response of the explants. BA and sucrose appear to act independently on different aspects of the competence of explants to respond to SIM during shoot initiation.
在辣椒（Capsicum annuum L. var. 'Sweet Banana'）上胚轴外植体启动阶段的初始培养基（SIM）中同时存在 6-苄基腺嘌呤（BA）和蔗糖时，发现这对于高频芽的形成是必需的。外植体在 SIM 上培养至少 8 天后，确定其芽的形成情况。在培养的第 6 天到第 20 天，去除外源性蔗糖对芽形成反应没有影响。BA 和蔗糖似乎在启动芽形成过程中，独立作用于外植体对 SIM 反应能力的不同方面。
