Department of Plant Biology, Royal Agricultural and Veterinary University of Copenhagen, Thorvaldsensvej 40, DK-1871, Frederiksberg C, Denmark.
Planta. 1992 Aug;188(1):78-84. doi: 10.1007/BF00198942.
Stimulation of leaf expansion by an exogenous cytokinin was studied in isolated leaf discs of sweet pepper with emphasis on the assimilate utilization of the tissue. Leaf discs were floated on solutions containing sucrose and plant growth regulators. Benzyladenine (BA) promoted the area expansion rate of the leaf discs. Sucrose at 100 mM resulted in increased area expansion rate compared with 10 mM sucrose. However, the increased sucrose concentration had no influence on the effect of BA. Over a period of 24 h, treatment with BA did not result in any change of sucrose uptake nor of the partitioning of assimilated carbon in the leaf discs. Neither did BA treatment affect the activity of acid invertase (EC 3.2.1.26) or pyrophosphate-dependent phosphofructokinase (EC 2.7.1.90) in the leaf discs. We conclude that the observed promotion of leaf area expansion by exogenous BA is not mediated through the uptake of sucrose or the carbohydrate metabolism of the leaf tissue.
外源细胞分裂素对甜椒离体叶片扩展的刺激作用研究，重点关注组织对同化产物的利用。叶片圆盘漂浮在含有蔗糖和植物生长调节剂的溶液上。苄基腺嘌呤（BA）促进叶片圆盘的面积扩展率。与 10mM 蔗糖相比，100mM 蔗糖导致面积扩展率增加。然而，增加的蔗糖浓度对 BA 的作用没有影响。在 24 小时的时间内，BA 的处理不会导致蔗糖摄取的任何变化，也不会导致同化碳在叶片圆盘内的分配发生变化。BA 处理也不会影响叶片圆盘中的酸性转化酶（EC 3.2.1.26）或焦磷酸依赖性磷酸果糖激酶（EC 2.7.1.90）的活性。我们得出结论，外源 BA 观察到的促进叶片面积扩展的作用不是通过蔗糖的摄取或叶片组织的碳水化合物代谢介导的。
