Laboratoire de Biologie Moléculaire des Relations Plantes-Microorganismes, INRA-CNRS, BP27, F-31326, Castanet-Tolosan Cedex, France.
Plant Cell Rep. 1996 Jan;15(5):305-10. doi: 10.1007/BF00232361.
Fertile and stable transgenic plants of the model legume Medicago truncatula Gaertn. were obtained through transformation of leaf tissue with the disarmed Agrobacterium tumefaciens strain LBA4404 and in vitro regeneration via somatic embryogenesis. An optimised transformation/regeneration protocol has been established for two genotypes of the cultivar Jemalong, including a previously described highly embryogenic line (Nolan et al. 1989, Plant Cell Rep. 8: 278-281). Using this protocol, transgenic plantlets were obtained within 4-10 months following cocultivation with Agrobacterium. We have introduced into M. truncatula a chimeric fusion between the early nodulin MtENOD12 promoter and the gus (β-glucuronidase) reporter gene, and shown that symbiosis-specific gene expression can be elicited in the roots of such transgenic plants following the addition of purified Rhizobium nodulation factors.
通过用去武装的根癌农杆菌 LBA4404 菌株和通过体细胞胚胎发生的体外再生转化叶组织，得到了模式豆科植物蒺藜苜蓿 Gaertn. 的可育且稳定的转基因植物。为包括先前描述的高胚胎发生系（Nolan 等人，1989 年，植物细胞报告，8:278-281）的栽培品种 Jemalong 的两个基因型建立了优化的转化/再生方案。使用该方案，在与根癌农杆菌共培养后 4-10 个月内获得了转基因苗。我们已经将早期结瘤素 MtENOD12 启动子和 gus（β-葡萄糖醛酸酶）报告基因之间的嵌合融合引入到蒺藜苜蓿中，并表明在添加纯化的根瘤菌结瘤因子后，这种转基因植物的根中可以引发共生特异性基因表达。
