Department of Plant Pathology, Georgia Station, University of Georgia, 1109 Experiment Street, 30223, Griffin, Georgia, USA.
Plant Cell Rep. 1996 May;15(9):653-7. doi: 10.1007/BF00231918.
Fertile transgenic plants of peanut (Arachis hypogaea L. cv. New Mexico Valencia A) were produced using an Agrobacterium-mediated transformation system. Leaf section explants were inoculated with A. tumefaciens strain EHA105 harboring the binary vector pBI121 containing the genes for β-glucuronidase (GUS) and neomycin phosphotransferase II (NPTII). Approximately 10% of the shoots regenerated on selection medium were GUS-positive. Five independent transformation events resulted in the production of 52 fertile transgenic peanut plants. On average, 240 d were required between seed germination for explant preparation and the production of mature t1 seed by T0 plants. Molecular analysis of transgenic plants confirmed the stable integration of the transgenes into the peanut genome. GUS expression segregated in a 3∶1 Mendelian ratio in most T1 generation plants.
利用农杆菌介导的转化系统，成功培育出了花生（Arachis hypogaea L. cv. New Mexico Valencia A）的可育转基因植株。叶片外植体与含有β-葡萄糖醛酸酶（GUS）和新霉素磷酸转移酶 II（NPTII）基因的二元载体 pBI121 的农杆菌菌株 EHA105 共培养。约 10%的再生芽在选择培养基上呈 GUS 阳性。5 个独立的转化事件导致产生了 52 株可育的转基因花生植株。平均而言，从外植体准备的种子发芽到 T0 植株产生成熟的 t1 种子需要 240 天。对转基因植物的分子分析证实了外源基因稳定整合到了花生基因组中。GUS 表达在大多数 T1 代植物中以 3∶1 的孟德尔比例分离。
