John Innes Centre for Plant Science Research, Colney Lane, NR4 7UH, Norwich, UK.
Plant Cell Rep. 1993 Jan;12(3):180-3. doi: 10.1007/BF00239102.
The lateral cotyledonary meristems present in germinating seed were inoculated with a non-oncogenic strain of A. tumefaciens carrying a gene conferring kanamycin resistance as a selectable marker and a β-glucuronidase sequence as a reporter gene. Kanamycin resistant plants were derived from the meristems and shown to be transformed on the basis of Southern blots, polymerase chain reaction analysis and tests for β-glucuronidase activity. The plants were fertile and tests of their progeny confirmed the transmission of integrated sequences through a sexual generation. This transformation method has the merit of an unlimited supply of material for inoculation and a relatively short time scale from inoculation to the production of rooted plants.
在萌发的种子中，侧生子叶分生组织被接种到携带赋予卡那霉素抗性的基因的非致癌根瘤农杆菌菌株中，该基因作为选择标记，β-葡萄糖醛酸酶序列作为报告基因。从分生组织中获得了卡那霉素抗性植物，并基于 Southern 印迹、聚合酶链反应分析和β-葡萄糖醛酸酶活性测试表明它们发生了转化。这些植物具有育性，对其后代的测试证实了整合序列通过有性世代的传递。这种转化方法具有接种材料供应无限和从接种到生根植物生产的时间尺度相对较短的优点。
