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细胞分裂素、生长素和脱落酸影响蔗糖代谢，有助于水稻（Oryza sativa L.）愈伤组织从头进行芽器官发生。

Cytokinin, auxin, and abscisic acid affects sucrose metabolism conduce to de novo shoot organogenesis in rice (Oryza sativa L.) callus.

作者信息

Lee Shiang-Ting, Huang Wen-Lii

机构信息

Department of Agronomy, National Chiayi University, Chiayi City 600, Taiwan.

出版信息

Bot Stud. 2013 Dec;54(1):5. doi: 10.1186/1999-3110-54-5. Epub 2013 Aug 13.

DOI:10.1186/1999-3110-54-5
PMID:28510848
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5383921/
Abstract

BACKGROUND

Shoot regeneration frequency in rice callus is still low and highly diverse among rice cultivars. This study aimed to investigate the association of plant hormone signaling and sucrose uptake and metabolism in rice during callus induction and early shoot organogenesis. The immatured seeds of two rice cultivars, Ai-Nan-Tsao 39 (ANT39) and Tainan 11 (TN11) are used in this study.

RESULTS

Callus formation is earlier, callus fresh weight is higher, but water content is significant lower in ANT39 than in TN11 while their explants are inoculated on callus induction medium (CIM). Besides, the regeneration frequency is prominently higher in ANT39 (~80%) compared to TN11 callus (0%). Levels of glucose, sucrose, and starch are all significant higher in ANT39 than in TN11 either at callus induction or early shoot organogenesis stage. Moreover, high expression levels of Cell wall-bound invertase 1, Sucrose transporter 1 (OsSUT1) and OsSUT2 are detected in ANT39 at the fourth-day in CIM but it cannot be detected in TN11 until the tenth-day. It suggested that ANT39 has higher callus growth rate and shoot regeneration ability may cause from higher activity of sucrose uptake and metabolism. As well, the expression levels of ORYZA SATIVA RESPONSE REGULATOR 1 (ORR1), PIN-formed 1 and Late embryogenesis-abundant 1, representing endogenous cytokinin, auxin and ABA signals, respectively, were also up-regulated in highly regenerable callus, ANT39, but only ORR1 was greatly enhanced in TN11 at the tenth-day in CIM.

CONCLUSION

Thus, phytohormone signals may affect sucrose metabolism to trigger callus initiation and further de novo shoot regeneration in rice culture.

摘要

背景

水稻愈伤组织的芽再生频率仍然较低，且在不同水稻品种间差异很大。本研究旨在探讨水稻愈伤组织诱导和早期芽器官发生过程中植物激素信号传导与蔗糖吸收及代谢之间的关联。本研究使用了两个水稻品种爱南早39（ANT39）和台南11号（TN11）的未成熟种子。

结果

当将它们的外植体接种在愈伤组织诱导培养基（CIM）上时，ANT39的愈伤组织形成更早，愈伤组织鲜重更高，但含水量显著低于TN11。此外，ANT39的再生频率（约80%）明显高于TN11愈伤组织（0%）。在愈伤组织诱导或早期芽器官发生阶段，ANT39中的葡萄糖、蔗糖和淀粉水平均显著高于TN11。此外，在CIM培养四天时，ANT39中检测到细胞壁结合型转化酶1、蔗糖转运蛋白1（OsSUT1）和OsSUT2的高表达水平，但直到第十天在TN11中才检测到。这表明ANT39具有较高的愈伤组织生长速率，其芽再生能力可能源于较高的蔗糖吸收和代谢活性。同样，分别代表内源性细胞分裂素、生长素和脱落酸信号的水稻应答调节因子1（ORR1）、PIN形成蛋白1和晚期胚胎丰富蛋白1的表达水平，在高再生能力的愈伤组织ANT39中也上调，但在CIM培养十天时，TN11中只有ORR1显著增强。

结论

因此，植物激素信号可能影响蔗糖代谢，从而触发水稻培养中愈伤组织的起始和进一步的从头芽再生。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3152/5383921/e7d1cc18b65f/40529_2013_Article_5_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3152/5383921/0d5e0a80d293/40529_2013_Article_5_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3152/5383921/32206ce1209c/40529_2013_Article_5_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3152/5383921/6dfb8ff97aa1/40529_2013_Article_5_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3152/5383921/f6a725748582/40529_2013_Article_5_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3152/5383921/e7d1cc18b65f/40529_2013_Article_5_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3152/5383921/0d5e0a80d293/40529_2013_Article_5_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3152/5383921/32206ce1209c/40529_2013_Article_5_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3152/5383921/6dfb8ff97aa1/40529_2013_Article_5_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3152/5383921/f6a725748582/40529_2013_Article_5_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3152/5383921/e7d1cc18b65f/40529_2013_Article_5_Fig5_HTML.jpg

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