Rozell B, Stenman G, Hansson H A, Dahl D, Hansson G K, Mark J
Acta Pathol Microbiol Immunol Scand A. 1985 Nov;93(6):335-43. doi: 10.1111/j.1699-0463.1985.tb03960.x.
Primary cultures of 9 benign human pleomorphic adenomas were analyzed by immunofluorescence and antibodies against the different intermediate filaments and microfilaments. The cultured cells were also cytogenetically characterized by G- and C-banding techniques. The adenoma cells expressed at least three different classes of intermediate filaments, viz. prekeratin, vimentin and glial fibrillary acidic protein (GFA). No correlation between chromosomal pattern and intermediate filament expression was found. The frequency of cells stained for each of the different antibodies varied considerably among the tumours. Prolonged culturing appeared to induce a reduction of cells positive for prekeratin. This was probably a true loss of antigens and not an effect of overgrowth of stromal cells. It is concluded that the cells studied, on the basis of several criteria, are of neoplastic origin. This in vitro system appears to be well suited for further histogenetic studies on pleomorphic adenomas.
采用免疫荧光法以及针对不同中间丝和微丝的抗体，对9例人良性多形性腺瘤的原代培养物进行了分析。同时，运用G显带和C显带技术对培养细胞进行了细胞遗传学特征分析。腺瘤细胞表达至少三类不同的中间丝，即前角蛋白、波形蛋白和胶质纤维酸性蛋白（GFA）。未发现染色体模式与中间丝表达之间存在相关性。不同肿瘤中，被不同抗体染色的细胞频率差异很大。长时间培养似乎会导致前角蛋白阳性细胞减少。这可能是抗原的真正丢失，而非基质细胞过度生长的影响。结论是，基于多项标准，所研究的细胞起源于肿瘤。该体外系统似乎非常适合对多形性腺瘤进行进一步的组织发生学研究。
