C3 植物中 NADP(+)-连接的苹果酸酶的细胞内位置。

Intracellular location of NADP(+)-linked malic enzyme in C 3 plants.

机构信息

Botany School, University of Cambridge, Downing Street, CB2 3EA, Cambridge, UK.

出版信息

Planta. 1991 Oct;185(3):362-7. doi: 10.1007/BF00201057.

DOI:10.1007/BF00201057

Abstract

The aim of this work was to determine the maximum catalytic activity and intracellular location of NADP(+)-linked malic enzyme (EC 1.1.1.40) in C3 plants. Appreciable activities, ranging from 80 to 712 nmol · (gFW)(-1) · min(-1), were found in a wide range of tissues (roots and leaves of Pisum sativum L., cotyledons of Cucurbit a pepo Alef., developing seeds of Brassica napus L., mesocarp of Persea americana Gaertn., and suspension cultures of Glycine max L.). Overall, activity showed a rough positive correlation with biosynthesis. Differential and density-gradient fractionation of extracts of the cotyledons of germinating marrow (C. pepo) and lysates of protoplasts of suspension cultures of G. showed that the enzyme had the same distribution as the plastid marker enzymes. It is suggested that in C3 plants NADP(+)-linked malic enzyme is confined to the plastids and involved in biosynthesis.

摘要

本工作旨在确定 NADP(+)-连接的苹果酸酶（EC 1.1.1.40）在 C3 植物中的最大催化活性和细胞内位置。在广泛的组织中（豌豆 Pisum sativum L. 的根和叶、南瓜 Cucurbit a pepo Alef. 的子叶、油菜 Brassica napus L. 的发育种子、鳄梨 Persea americana Gaertn. 的中果皮和大豆 Glycine max L. 的悬浮培养物）发现了相当大的活性，范围从 80 到 712 nmol·(gFW)(-1)·min(-1)。总体而言，活性与生物合成呈正相关。发芽骨髓（C. pepo）子叶提取物和悬浮培养原生质体裂解物的差异和密度梯度分级表明，该酶与质体标记酶具有相同的分布。因此，在 C3 植物中，NADP(+)-连接的苹果酸酶局限于质体并参与生物合成。