Fushimi T, Umeda M, Shimazaki T, Kato A, Toriyama K, Uchimiya H
Institute of Molecular and Cellular Biosciences, University of Tokyo, Japan.
Plant Mol Biol. 1994 Mar;24(6):965-7. doi: 10.1007/BF00014450.
We have isolated a rice cDNA clone that is homologous to the gene for the maize NADP-dependent malic enzyme (EC 1.1.1.40; NADP-ME). The deduced amino acid sequence coded for by the cDNA indicates a high level of homology to chloroplast type NADP-ME, including a transit peptide with pronounced hydrophobic properties at the amino terminus. Northern blot analysis indicates that the expression of this gene is regulated by external stress such as submergence.
我们分离出了一个水稻cDNA克隆,它与玉米依赖NADP的苹果酸酶(EC 1.1.1.40;NADP-ME)的基因同源。该cDNA编码的推导氨基酸序列显示与叶绿体型NADP-ME具有高度同源性,包括在氨基末端具有明显疏水特性的转运肽。Northern印迹分析表明,该基因的表达受诸如淹水等外部胁迫的调控。