Department of Otolaryngology, Guang Ming New District People's Hospital of Shenzhen, Song Bai Road 339#, Shenzhen, Guang Dong TX 518106, PR China.
Department of Otolaryngology, Nanshan People's Hospital of Shenzhen, Tao Yuan Road 89#, Shenzhen, Guang Dong TX 518052, PR China.
Cancer Cell Int. 2013 Nov 5;13(1):109. doi: 10.1186/1475-2867-13-109.
The transcription factor SOX11 is one of members of the SRY box-containing (SOX) family emerging as important transcriptional regulators. In recent years, up-regulation of SOX11 has been detected in various types of solid tumors. In this study, the effects of promoter methylation of the SOX11 gene on SOX11 expression and cell growth and invasion of nasopharyngeal carcinoma were investigated.
In this study,methylation-specific PCR and real time quantitative PCR have been applied to investigate the effect of promoter methylation of the SOX11 gene on SOX11 expression in the nasopharyngeal carcinoma and chronic inflammation tissues. The nasopharyngeal carcinoma cell line (CNE2) was treated with 5-aza-2'-deoxycytidine. The effect of promoter methylation of SOX11 on growth and invasion of nasopharyngeal carcinoma cells was detected with MTT test and Boyden chamber Matrigel invasion assay.
No or weak expression of SOX11 mRNA was detected in the nasopharyngeal carcinoma tissues of SOX11 gene promoter methylation. Strong expression of SOX11 mRNA was detected in the nasopharyngeal carcinoma tissues of SOX11 gene promoter unmethylation and chronic inflammation tissues of pharynx nasalis. SOX11 mRNA and protein were re-expressed, SOX11 gene was demethylated, and growth and invasion of cells were inhibited in CNE2 cell line after 5-aza-2'-deoxycytidine treatment.
The results of the study indicate that expression of SOX11 mRNA and protein were related to SOX11 gene methylation status. SOX11 gene methylation may be plays a role in growth and invasion of nasopharyngeal carcinoma cells.
转录因子 SOX11 是 SRY 盒包含 (SOX) 家族的成员之一，作为重要的转录调节因子而出现。近年来，SOX11 的上调已在各种类型的实体瘤中被检测到。在这项研究中，研究了 SOX11 基因启动子甲基化对鼻咽癌中 SOX11 表达和细胞生长及侵袭的影响。
本研究应用甲基化特异性 PCR 和实时定量 PCR 研究了 SOX11 基因启动子甲基化对鼻咽癌和慢性炎症组织中 SOX11 表达的影响。用 5-氮杂-2'-脱氧胞苷处理鼻咽癌细胞系（CNE2）。用 MTT 试验和 Boyden 室 Matrigel 侵袭试验检测 SOX11 启动子甲基化对鼻咽癌细胞生长和侵袭的影响。
SOX11 基因启动子甲基化的鼻咽癌组织中检测到 SOX11 mRNA 表达缺失或微弱，SOX11 基因启动子非甲基化的鼻咽癌组织和鼻咽部慢性炎症组织中检测到 SOX11 mRNA 表达较强。SOX11 mRNA 和蛋白重新表达，CNE2 细胞系经 5-氮杂-2'-脱氧胞苷处理后，SOX11 基因去甲基化，细胞生长和侵袭受到抑制。
研究结果表明，SOX11 mRNA 和蛋白的表达与 SOX11 基因甲基化状态有关。SOX11 基因甲基化可能在鼻咽癌细胞的生长和侵袭中起作用。
