Department of Biology, Sookmyung Women's University, 140-742, Seoul.
Microb Ecol. 1993 Nov;26(3):219-26. doi: 10.1007/BF00176954.
The OCT plasmid from Pseudomonas maltophilia N246-1 was transferred to Rhodopseudomonas sphaeroides M1 with very low frequency (1.4-1.9 × 10(-5) per recipient cell at pH 7-8 for a 3-hour reaction time). P. maltophilia N246-1 was able to utilize C8-C14 of n-alkanes, whereas R. gas-liquid chromatography determined that the broad range of carbon numbers of n-alkanes in crude oil was remarkably degraded by the transconjugant, R. sphaeroides M1-C1, compared with donor strain N246-1. The fact that donor and transconjugant strains simultaneously lost the capacity to utilize n-alkanes on L-broth medium suggests that the OCT plasmids are unstable. It was found that the OCT plasmid of P. maltophilia N246 was incompatible with the IncP-2 group of P. aeruginosa KCTC 11245.
从假单胞菌 N246-1 中提取的 OCT 质粒被转移到球形红假单胞菌 M1 中，其转移频率非常低（在 pH 值为 7-8 时，反应时间为 3 小时，每受体细胞的转移频率为 1.4-1.9×10(-5)）。假单胞菌 N246-1 能够利用 C8-C14 的正构烷烃，而气相色谱法确定，与供体菌株 N246-1 相比，原油中宽范围的正构烷烃碳数被转导子球形红假单胞菌 M1-C1 显著降解。供体和转导子菌株同时失去在 L-肉汤培养基中利用正构烷烃的能力这一事实表明，OCT 质粒是不稳定的。发现假单胞菌 N246 的 OCT 质粒与铜绿假单胞菌 KCTC 11245 的 IncP-2 组是不相容的。
