Laboratoire de Physiologie Cellulaire et Morphogenèse Végétales, Université des Sciences et Technologies de Lille-Flandres-Artois, 59655, Villeneuve d'Ascq cedex, France.
Theor Appl Genet. 1993 Nov;87(3):347-52. doi: 10.1007/BF01184922.
Male-sterile chicory plants were obtained by fusion of chicory mesophyll protoplasts and hypocotyl protoplasts derived from male-sterile sunflower plants. The protoplasts of both species were fused by the PEG method and the products were selected manually and cultivated at very low density in a liquid medium. Three to twenty percent of the heterokaryocytes divided and evolved into microcalli, then into calli where budding could be induced. The mitochondrial genome of ten male-sterile or totally sterile plants was studied. Restriction endonuclease profiles of mitochondrial DNA and molecular hybridization with specific genes of the mitochondrial genome used as probes indicated that mitochondrial DNA rearrangement had occurred between sunflower and chicory and the intensity of the rearrangements correlated with the degree of sterility of the different plants.
雄性不育菊苣植株是通过融合菊苣叶肉原生质体和来自雄性不育向日葵植株的下胚轴原生质体而获得的。两种物种的原生质体通过 PEG 方法融合，产物通过手动选择并在非常低的密度下在液体培养基中培养。百分之三到二十的异核细胞分裂并进化为微愈伤组织，然后形成可以诱导出芽的愈伤组织。对十株雄性不育或完全不育的植物的线粒体基因组进行了研究。线粒体 DNA 的限制性内切酶图谱和用作为探针的线粒体基因组的特定基因的分子杂交表明，在向日葵和菊苣之间发生了线粒体 DNA 重排，重排的强度与不同植物的不育程度相关。
