不同农杆菌菌株对曼陀罗毛状根形成的毒力。

Virulence of different Agrobacterium strains on hairy root formation of Hyoscyamus muticus.

机构信息

Pharmacognosy Division, Department of Pharmacy, University of Helsinki, Fabianinkatu 35, P.O. Box 15, FIN-00014, Finland.

出版信息

Plant Cell Rep. 1995 Jan;14(4):236-40. doi: 10.1007/BF00233640.

DOI:10.1007/BF00233640

PMID:24190302

Abstract

Hyoscyamus muticus accession was evaluated for its response to inoculation with Agrobacterium rhizogenes strains LBA9402, A4, 15834, and Agrobacterium tumefaciens strain C58 CI pRT GUS104. The Agrobacterium strain used for the transformation has a significant influence on the phenotype of the clone as well as on the growth rate and hyoscyamine production of these root culture clones. The most virulent strains were C58 CI pRT GUS104 and LBA9402. More roots were obtained on LSO medium than on B50 medium. Acetosyringone addition and the time from wounding affected root formation. The alkaloid content was highest in clones C58 and A4 (≈90mg·l(-1)). There are great differences between individual hairy root clones, and hence they are not as uniform as has often been speculated. The Agrobacterium strain used for the transformation has a great influence in this respect.

摘要

黄花曼陀罗接入体被评价对根癌农杆菌菌株 LBA9402、A4、15834 和根瘤农杆菌菌株 C58 CI pRT GUS104 的接种反应。用于转化的农杆菌菌株对克隆的表型以及这些根培养克隆的生长速度和天仙子胺产量有显著影响。最毒力的菌株是 C58 CI pRT GUS104 和 LBA9402。在 LSO 培养基上获得的根比在 B50 培养基上获得的根多。乙酰丁香酮的添加和创伤后的时间影响根的形成。在克隆 C58 和 A4 中生物碱含量最高（≈90mg·l(-1)）。个别发根克隆之间存在很大差异，因此它们并不像人们经常推测的那样均匀。在这方面，用于转化的农杆菌菌株有很大的影响。