Laboratoire Agronomie et Environenment, ENSAIA-INRA, 2, Avenue de la Forêt de Haye, 54500, Vandoeuvre-lès-Nancy, France.
Plant Cell Rep. 1992 Jul;11(8):424-7. doi: 10.1007/BF00234375.
Eight Psoralea species (Leguminosae) were inoculated with Agrobacterium rhizogenes, strains 8196 and 9402. Hairy roots were only induced by strain 9402. Attention was focussed on Psoralea lachnostachys. Transformed roots grew very rapidly in Gamborg B5 liquid medium with a doubling time of the culture of 38 hours. Whatever the culture conditions, the two furanocoumarins usually found in roots of Psoralea plants, psoralen and angelicin, were not detected in cultured transformed and non transformed roots even when some chitosan was added to the medium. However, 669 μg.g(-1) dry matter of psoralen and 215 μg.g(-1) dry matter of angelicin were found in roots from soil grown plants. A possible translocation of these compounds from the aerial parts to the roots is suggested.
八种补骨脂属植物（豆科）被发根农杆菌菌株 8196 和 9402 接种。只有菌株 9402 才能诱导出发根。我们对补骨脂属植物中的 Psoralea lachnostachys 进行了重点研究。转化根在 Gamborg B5 液体培养基中生长非常迅速，培养物的倍增时间为 38 小时。无论培养条件如何，在培养的转化根和非转化根中都没有检测到通常存在于补骨脂植物根中的两种呋喃香豆素，即补骨脂素和花椒毒素，即使在培养基中添加了一些壳聚糖也是如此。然而，在土壤中生长的植物的根中发现了 669μg.g(-1)干物质的补骨脂素和 215μg.g(-1)干物质的花椒毒素。这表明这些化合物可能从地上部分转移到根部。
