Neuroscience Research Australia, Barker Street, Randwick, NSW 2031, Australia ; Prince of Wales Clinical School, The University of New South Wales, Randwick, NSW 2031, Australia.
Mediators Inflamm. 2013;2013:890706. doi: 10.1155/2013/890706. Epub 2013 Sep 28.
Cytokine concentrations are thought to be affected by methods of sampling and processing and by storage conditions. In this study we compared 17 cytokine concentrations obtained from plasma and serum at baseline and after a controlled thaw condition. We found that absolute agreement was poor between concentrations of cytokines in plasma and serum, except for MIP1 β . A thaw condition significantly changed the concentrations of most cytokines, but serum appeared less affected by this than plasma was. Closer examination using Bland-Altman analyses revealed that for each comparison, agreement was moderately good for many cytokine concentrations. This is important because measures of agreement must be interpreted based on the required precision, which may differ between clinical and research demands. We also identified that for some cytokines, the relationship between serum and plasma is affected by concentration, thus advocating for the use of appropriate methods when performing such comparisons in studies such as systematic reviews and meta-analyses.
细胞因子浓度被认为受到采样和处理方法以及储存条件的影响。在这项研究中，我们比较了基线和受控解冻条件下从血浆和血清中获得的 17 种细胞因子浓度。我们发现，除了 MIP1β 之外，血浆和血清中细胞因子的浓度之间的一致性很差。解冻条件显著改变了大多数细胞因子的浓度，但血清似乎比血浆受影响小。使用 Bland-Altman 分析进行更仔细的检查后发现，对于每种比较，许多细胞因子浓度的一致性都很好。这很重要，因为一致性的衡量标准必须根据所需的精度进行解释，而这可能因临床和研究需求的不同而有所差异。我们还发现，对于某些细胞因子，血清和血浆之间的关系受到浓度的影响，因此在进行系统评价和荟萃分析等研究时，使用适当的方法进行此类比较很重要。
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