Müller W
Eur J Biochem. 1986 Feb 17;155(1):213-22. doi: 10.1111/j.1432-1033.1986.tb09479.x.
New phase supports for liquid-liquid partition chromatography, using aqueous poly(ethyleneglycol)-dextran systems have been developed by grafting linear polyacrylamide chains on to premanufactured chromatographic supports carrying primary or secondary aliphatic hydroxyl functions on their surface. Columns prepared from such supports have a higher binding capacity for the dextran-rich stationary phase and much higher performances than columns prepared from cellulose, the previously used phase support for this system. Test separations of DNA restriction fragments, ranging from 11 base pairs to 3829 base pairs, document a high resolution for DNA fragments larger than 200 base pairs.
通过将线性聚丙烯酰胺链接枝到预先制造的、表面带有伯或仲脂肪族羟基官能团的色谱载体上，已开发出用于液-液分配色谱的新型水相聚乙二醇-葡聚糖系统载体。由这种载体制备的柱对富含葡聚糖的固定相具有更高的结合能力，并且比由纤维素制备的柱具有更高的性能，纤维素是该系统先前使用的相载体。对11个碱基对至3829个碱基对的DNA限制性片段进行的测试分离表明，对于大于200个碱基对的DNA片段具有高分辨率。
