一种用于通过高效液相色谱法分离DNA限制性片段的新型填料。
A new packing for separation of DNA restriction fragments by high performance liquid chromatography.
作者信息
Kato Y, Sasaki M, Hashimoto T, Murotsu T, Fukushige S, Matsubara K
出版信息
J Biochem. 1984 Jan;95(1):83-6. doi: 10.1093/oxfordjournals.jbchem.a134606.
Abstract
TSK-GEL SW was found to be useful as a packing in high performance liquid chromatography for the separation of double-stranded DNA restriction fragments. DNA fragments smaller than 300 base pairs were separated as discrete peaks depending solely upon difference in chain length. The recovery of DNA fragments was higher than 90%.
摘要
发现TSK - GEL SW作为高效液相色谱中的填料，可用于分离双链DNA限制性片段。小于300个碱基对的DNA片段仅根据链长差异被分离为离散的峰。DNA片段的回收率高于90%。
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