• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

通过液-液色谱法对DNA片段进行尺寸分级分离。

Size fractionation of DNA fragments by liquid-liquid chromatography.

作者信息

Müller W, Schuetz H J, Guerrier-Takada C, Cole P E, Potts R

出版信息

Nucleic Acids Res. 1979 Dec 20;7(8):2483-99. doi: 10.1093/nar/7.8.2483.

DOI:10.1093/nar/7.8.2483
PMID:160546
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC342398/
Abstract

A method for the fractionation of double-stranded DNA fragments from 150 to 22000 b.p. in size by liquid-liquid chromatography is described. The procedure makes use of the fact that the partitioning of DNA in a polyethylene glycol-dextran system is size dependent and can be altered by alkali metal cations. Cellulose or celite are used as supports for the stationary, dextran-rich phase. Examples show the fractionation of digests of T7 DNA produced by Dpn II and Hind II restriction endonulceases as well as lambda DNA digests produced by Hind III and Eco RI restriction endonucleases.

摘要

本文描述了一种通过液-液色谱法分离大小在150至22000碱基对之间的双链DNA片段的方法。该方法利用了DNA在聚乙二醇-葡聚糖系统中的分配取决于大小且可被碱金属阳离子改变这一事实。纤维素或硅藻土用作富含葡聚糖的固定相的支持物。实例展示了由Dpn II和Hind II限制性内切酶产生的T7 DNA消化产物以及由Hind III和Eco RI限制性内切酶产生的λ DNA消化产物的分级分离。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2fd1/342398/5b5196561cba/nar00461-0415-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2fd1/342398/0d4d00383e91/nar00461-0407-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2fd1/342398/73ca168386b2/nar00461-0411-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2fd1/342398/7143ee7fc6d1/nar00461-0413-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2fd1/342398/e94eae7d4358/nar00461-0413-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2fd1/342398/c1bdbf156808/nar00461-0414-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2fd1/342398/5b5196561cba/nar00461-0415-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2fd1/342398/0d4d00383e91/nar00461-0407-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2fd1/342398/73ca168386b2/nar00461-0411-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2fd1/342398/7143ee7fc6d1/nar00461-0413-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2fd1/342398/e94eae7d4358/nar00461-0413-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2fd1/342398/c1bdbf156808/nar00461-0414-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2fd1/342398/5b5196561cba/nar00461-0415-a.jpg

相似文献

1
Size fractionation of DNA fragments by liquid-liquid chromatography.通过液-液色谱法对DNA片段进行尺寸分级分离。
Nucleic Acids Res. 1979 Dec 20;7(8):2483-99. doi: 10.1093/nar/7.8.2483.
2
New phase supports for liquid-liquid partition chromatography of biopolymers in aqueous poly(ethyleneglycol)-dextran systems. Synthesis and application for the fractionation of DNA restriction fragments.新型固定相用于水性聚乙二醇-葡聚糖体系中生物聚合物的液-液分配色谱。DNA限制性片段分离的合成与应用。
Eur J Biochem. 1986 Feb 17;155(1):213-22. doi: 10.1111/j.1432-1033.1986.tb09479.x.
3
[The use of fractionation in a polyethylene glycol-dextran two-phase system for the testing and purification of restriction endonucleases].[聚乙二醇-葡聚糖双相系统中分级分离在限制性内切核酸酶检测与纯化中的应用]
Prikl Biokhim Mikrobiol. 1987 Jan-Feb;23(1):84-92.
4
Mechanism of DNA hydrodynamic separation in chromatography.色谱中DNA流体动力学分离的机制。
Anal Chem. 2000 Feb 15;72(4):853-7. doi: 10.1021/ac990841s.
5
A simple and efficient procedure for the isolation of high-quality phage lambda DNA using a DEAE-cellulose column.一种使用二乙氨基乙基纤维素柱分离高质量λ噬菌体DNA的简单高效方法。
Anal Biochem. 1988 Feb 1;168(2):324-31. doi: 10.1016/0003-2697(88)90325-9.
6
A simple and rapid method for the isolation of plasmid and lambda phage DNAs.一种分离质粒和λ噬菌体DNA的简单快速方法。
Nucleic Acids Res. 1989 Dec 11;17(23):10129. doi: 10.1093/nar/17.23.10129.
7
Electrophoretic mobility of lambda phage HIND III and HAE III DNA fragments in agarose gels: a detailed study.λ噬菌体HIND III和HAE III DNA片段在琼脂糖凝胶中的电泳迁移率：一项详细研究。
Biopolymers. 1987 May;26(5):727-42. doi: 10.1002/bip.360260512.
8
Preparative fractionation of DNA restriction fragments by high pressure column chromatography on RPC-5.通过RPC - 5柱上高压柱色谱法对DNA限制片段进行制备性分级分离。
Nucleic Acids Res. 1976 Oct;3(10):2575-92. doi: 10.1093/nar/3.10.2575.
9
Large-scale purification of two forms of active lac operator from plasmids.从质粒中大规模纯化两种活性乳糖操纵子形式。
Biochim Biophys Acta. 1980;606(1):113-24. doi: 10.1016/0005-2787(80)90103-3.
10
Quick method for high yields of lambda bacteriophage DNA.高产λ噬菌体DNA的快速方法。
Nucleic Acids Res. 1990 Apr 11;18(7):1923. doi: 10.1093/nar/18.7.1923.

本文引用的文献

1
Fractionation of nucleic acids in aqueous polymer tow-phase systems.核酸在水性聚合物双相系统中的分级分离。
J Mol Biol. 1961 Dec;3:727-40. doi: 10.1016/s0022-2836(61)80077-6.
2
A method for extracting purified DNA or protein-DNA complex from escherichia coli.一种从大肠杆菌中提取纯化DNA或蛋白质-DNA复合物的方法。
Eur J Biochem. 1967 Dec;3(1):33-41. doi: 10.1111/j.1432-1033.1967.tb19496.x.
3
Chain length determination of small double- and single-stranded DNA molecules by polyacrylamide gel electrophoresis.通过聚丙烯酰胺凝胶电泳测定小双链和单链DNA分子的链长
Biochemistry. 1975 Aug 26;14(17):3787-94. doi: 10.1021/bi00688a010.
4
The biological activity of bacteriophage DNA, prepared by the cationic detergent dilution technique.通过阳离子去污剂稀释技术制备的噬菌体DNA的生物活性。
Nucleic Acids Res. 1975 Mar;2(3):347-51. doi: 10.1093/nar/2.3.347.
5
Restriction enzyme cleavage mapping of T7 virus early region.T7病毒早期区域的限制性内切酶切割图谱分析
Mol Gen Genet. 1978 Jul 4;162(3):329-39. doi: 10.1007/BF00268859.