通过液-液色谱法对DNA片段进行尺寸分级分离。

Size fractionation of DNA fragments by liquid-liquid chromatography.

作者信息

Müller W, Schuetz H J, Guerrier-Takada C, Cole P E, Potts R

出版信息

Nucleic Acids Res. 1979 Dec 20;7(8):2483-99. doi: 10.1093/nar/7.8.2483.

DOI:10.1093/nar/7.8.2483

PMID:160546

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC342398/

Abstract

A method for the fractionation of double-stranded DNA fragments from 150 to 22000 b.p. in size by liquid-liquid chromatography is described. The procedure makes use of the fact that the partitioning of DNA in a polyethylene glycol-dextran system is size dependent and can be altered by alkali metal cations. Cellulose or celite are used as supports for the stationary, dextran-rich phase. Examples show the fractionation of digests of T7 DNA produced by Dpn II and Hind II restriction endonulceases as well as lambda DNA digests produced by Hind III and Eco RI restriction endonucleases.

摘要

本文描述了一种通过液-液色谱法分离大小在150至22000碱基对之间的双链DNA片段的方法。该方法利用了DNA在聚乙二醇-葡聚糖系统中的分配取决于大小且可被碱金属阳离子改变这一事实。纤维素或硅藻土用作富含葡聚糖的固定相的支持物。实例展示了由Dpn II和Hind II限制性内切酶产生的T7 DNA消化产物以及由Hind III和Eco RI限制性内切酶产生的λ DNA消化产物的分级分离。