Brooks Eric R, Wallingford John B
Section of Molecular Cell and Developmental Biology and the Institute for Cellular and Molecular Biology, The University of Texas at Austin, Patterson Labs, 2401 Speedway, Austin, TX 78712, USA.
Section of Molecular Cell and Developmental Biology and the Institute for Cellular and Molecular Biology, The University of Texas at Austin, Patterson Labs, 2401 Speedway, Austin, TX 78712, USA ; The Howard Hughes Medical Institute, Chevy Chase, USA.
Cilia. 2013 Oct 7;2:13. doi: 10.1186/2046-2530-2-13. eCollection 2013.
Cilia are small, microtubule-based protrusions important for development and homeostasis. We recently demonstrated that the planar cell polarity effector protein Fuz is a critical regulator of axonemal intraflagellar transport dynamics and localization. Here, we report our findings on the role of the small GTPase Rsg1, a known binding partner of Fuz, and its role in the dynamics and cytoplasmic localization of intraflagellar transport proteins.
We find that Rsg1 loss of function leads to impaired axonemal IFT dynamics in multiciliated cells. We further show that Rsg1 is required for appropriate cytoplasmic localization of the retrograde IFT-A protein IFT43. Finally, we show that Rsg1 governs the apical localization of basal bodies, the anchoring structures of cilia.
Our data suggest that Rsg1 is a regulator of multiple aspects of ciliogenesis, including apical trafficking of basal bodies and the localization and dynamics intraflagellar transport proteins.
纤毛是基于微管的小突起，对发育和体内平衡很重要。我们最近证明，平面细胞极性效应蛋白Fuz是轴丝内鞭毛运输动力学和定位的关键调节因子。在这里，我们报告了关于小GTP酶Rsg1的作用的研究结果，Rsg1是Fuz已知的结合伙伴，及其在鞭毛内运输蛋白的动力学和细胞质定位中的作用。
我们发现Rsg1功能丧失导致多纤毛细胞中轴丝IFT动力学受损。我们进一步表明，Rsg1是逆向IFT-A蛋白IFT43在细胞质中正确定位所必需的。最后，我们表明Rsg1控制基体（纤毛的锚定结构）的顶端定位。
我们的数据表明，Rsg1是纤毛发生多个方面的调节因子，包括基体的顶端运输以及鞭毛内运输蛋白的定位和动力学。
