Balan Tavamani, Mohd Sani Mohd Hijaz, Suppaiah Velan, Mohtarrudin Norhafizah, Suhaili Zarizal, Ahmad Zuraini, Zakaria Zainul Amiruddin
Department of Biomedical Sciences .
Pharm Biol. 2013 Nov 5. doi: 10.3109/13880209.2013.839713.
Abstract Context: Muntingia calabura L. (Muntingiaceae) is a native plant species of the American continent and is widely cultivated in warm areas in Asia, including Malaysia. The plant is traditionally used to relieve pain from gastric ulcers. Objective: This study was designed to determine the antiulcer activity of a methanol extract of M. calabura leaves (MEMC) and the possible mechanisms of action involved. Materials and methods: An acute toxicity study was conducted using a single oral dose of 2000 mg/kg MEMC. The antiulcer activity of MEMC was evaluated in absolute ethanol- and indomethacin-induced gastric ulcer rat models. MEMC was administered orally (dose range 25-500 mg/kg) to rats fasted for 24 h. The animals were pretreated with N-nitro-l-arginine methyl esters (l-NAME) or N-ethylmaleimide (NEM) prior to MEMC treatment to assess the possible involvement of endogenous nitric oxide (NO) and nonprotein sulfhydryl (NP-SH) compounds in the gastroprotective effect of MEMC. Results: As the administered dose did not cause toxicity in the rats, the oral median lethal dose (LD) of MEMC was >2000 mg/kg in rats. MEMC exerted significant (p < 0.001) gastroprotective activity in the ethanol- and indomethacin-induced ulcer models dose-dependently. Histological evaluation supported the observed antiulcer activity of MEMC. l-NAME and NEM pretreatment significantly (p < 0.05) reversed and abolished the gastroprotective effect of MEMC, respectively. Discussion and conclusion: The results obtained indicate that MEMC has significant antiulcer activity that might involve the participation of endogenous NO and NP-SH compounds. These findings provide new pharmacological information regarding the potential use of M. calabura.
摘要 背景：黄金树（锦葵科）是美洲的本土植物物种，在亚洲温暖地区广泛种植，包括马来西亚。该植物传统上用于缓解胃溃疡疼痛。目的：本研究旨在确定黄金树叶甲醇提取物（MEMC）的抗溃疡活性及其可能的作用机制。材料与方法：采用单次口服2000mg/kg MEMC进行急性毒性研究。在绝对乙醇和吲哚美辛诱导的胃溃疡大鼠模型中评估MEMC的抗溃疡活性。将MEMC口服给予禁食24小时的大鼠（剂量范围25-500mg/kg）。在MEMC治疗前用N-硝基-L-精氨酸甲酯（L-NAME）或N-乙基马来酰亚胺（NEM）预处理动物，以评估内源性一氧化氮（NO）和非蛋白巯基（NP-SH）化合物是否可能参与MEMC的胃保护作用。结果：由于给药剂量未对大鼠造成毒性，MEMC在大鼠中的口服半数致死剂量（LD）>2000mg/kg。MEMC在乙醇和吲哚美辛诱导的溃疡模型中具有显著的（p<0.001）剂量依赖性胃保护活性。组织学评估支持了观察到的MEMC的抗溃疡活性。L-NAME和NEM预处理分别显著（p<0.05）逆转和消除了MEMC的胃保护作用。讨论与结论：所得结果表明，MEMC具有显著的抗溃疡活性，可能涉及内源性NO和NP-SH化合物的参与。这些发现为黄金树的潜在用途提供了新的药理学信息。
